論文 - 詳細
| RRC ID | 41890 |
|---|---|
| 著者 | Kim H, Arakawa H, Osada T, Ikai A. |
| タイトル | Quantification of cell adhesion force with AFM: distribution of vitronectin receptors on a living MC3T3-E1 cell. |
| ジャーナル | Ultramicroscopy |
| Abstract |
Distribution of vitronectin (VN) receptors on a living murine osteoblastic cell was successfully measured by atomic force microscopy (AFM). First, the distribution of the integrin beta(5) subunit which constitutes a part of the VN receptor on the cell was confirmed by conventional immunohistochemistry after fixing the cell. To visualize the distribution of the receptor on a living cell by an independent and potentially a more quantitative method, the AFM was used with a microbead attached to the cantilever tip to increase the area of contact and VN was immobilized on the microbead. Force measurements were then performed over a large area of a living murine osteoblastic cell using the microbead covered with VN. |
| 巻・号 | 97(1-4) |
| ページ | 359-63 |
| 公開日 | 2003-1-1 |
| DOI | 10.1016/S0304-3991(03)00061-5 |
| PII | S0304-3991(03)00061-5 |
| PMID | 12801689 |
| MeSH | Animals Cell Adhesion Cell Line Immunohistochemistry Integrin beta Chains / metabolism Mice Microscopy, Atomic Force / methods* Osteoblasts / metabolism Osteoblasts / ultrastructure* Receptors, Vitronectin / metabolism Receptors, Vitronectin / ultrastructure* Vitronectin / metabolism* |
| IF | 2.452 |
| 引用数 | 54 |
| WOS 分野 | MICROSCOPY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |