論文 - 詳細
| RRC ID | 41905 |
|---|---|
| 著者 | Hino M, Kijima-Suda I, Nagai Y, Hosoya H. |
| タイトル | Glycosylation of the alpha and beta tubulin by sialyloligosaccharides. |
| ジャーナル | Zoolog Sci |
| Abstract |
To examine whether alpha and beta tubulin are glycoproteins, we used a pyridylamino labeling method and a monoclonal antibody, SG3-1, raised against NeuAcalpha2-3Gal structure. Alpha and beta tubulin from both pig brain and HeLa cells were positive for the SG3-1 antibody by immunoblot assay. Sialidase treatment reduced the reactivity of the SG3-1 antibody to alpha and beta tubulin molecules. N-linked oligosaccharide analysis also showed that alpha and beta tubulin are glycosylated. Moreover, immunofluorescence analysis showed that the filamentous structure recognized by the SG3-1 antibody was overlapped with microtubules, especially in the vicinity of the nucleus. These results indicate that alpha and beta tubulin are glycosylated with sialyloligosaccharides. |
| 巻・号 | 20(6) |
| ページ | 709-15 |
| 公開日 | 2003-6-1 |
| DOI | 10.2108/zsj.20.709 |
| PMID | 12832821 |
| MeSH | Animals Antibodies, Monoclonal Brain Glycosylation HeLa Cells Humans Immunoblotting Oligosaccharides / chemistry Oligosaccharides / pharmacology* Swine Tubulin / metabolism* |
| IF | 0.843 |
| 引用数 | 18 |
| WOS 分野 | ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |