RRC ID 41912
Author Takahashi T, Haraguchi K, Chiba S, Yasukawa M, Shibata Y, Hirai H.
Title Valpha24+ natural killer T-cell responses against T-acute lymphoblastic leukaemia cells: implications for immunotherapy.
Journal Br J Haematol
Abstract Human Valpha24+ natural killer T (NKT) cells correspond to mouse Valpha14+ NKT cells, both cell types use an invariant T-cell receptor-alpha chain and are activated by glycolipids in a CD1d-dependent manner. Mouse Valpha14+ NKT cells have been reported to have an antitumour effect in vivo. Human Valpha24+ NKT cells can kill a proportion of tumour cells in a CD1d-dependent manner in vitro. We report here that many human leukaemic T-cell lines express CD1d and can be directly killed by Valpha24+ NKT cells. This killing activity was enhanced in the presence of alpha-galactosylceramide (alpha-GalCer), a ligand of Valpha24+ NKT cells. Moreover, primary leukaemic T cells from five of eight T-cell acute lymphoblastic leukaemia (T-ALL) patients expressed CD1d and were good targets of Valpha24+ NKT cells. This cytotoxicity was increased in the presence of alpha-GalCer. Our results suggest that T-ALL is a good candidate for Valpha24+ NKT-cell-based immuno-cell therapy.
Volume 122(2)
Pages 231-9
Published 2003-7-1
DOI 10.1046/j.1365-2141.2003.04429.x
PII 4429
PMID 12846891
MeSH Antigens, CD1 / analysis Cytokines / immunology Cytotoxicity Tests, Immunologic Humans Immunophenotyping Immunotherapy, Adoptive / methods* Killer Cells, Natural / immunology* Leukemia-Lymphoma, Adult T-Cell / immunology* Lymphocyte Activation Receptors, Antigen, T-Cell, alpha-beta / immunology* Tumor Cells, Cultured
IF 5.518
Times Cited 25
Human and Animal Cells K562(RCB0027)