| RRC ID |
41912
|
| Author |
Takahashi T, Haraguchi K, Chiba S, Yasukawa M, Shibata Y, Hirai H.
|
| Title |
Valpha24+ natural killer T-cell responses against T-acute lymphoblastic leukaemia cells: implications for immunotherapy.
|
| Journal |
Br J Haematol
|
| Abstract |
Human Valpha24+ natural killer T (NKT) cells correspond to mouse Valpha14+ NKT cells, both cell types use an invariant T-cell receptor-alpha chain and are activated by glycolipids in a CD1d-dependent manner. Mouse Valpha14+ NKT cells have been reported to have an antitumour effect in vivo. Human Valpha24+ NKT cells can kill a proportion of tumour cells in a CD1d-dependent manner in vitro. We report here that many human leukaemic T-cell lines express CD1d and can be directly killed by Valpha24+ NKT cells. This killing activity was enhanced in the presence of alpha-galactosylceramide (alpha-GalCer), a ligand of Valpha24+ NKT cells. Moreover, primary leukaemic T cells from five of eight T-cell acute lymphoblastic leukaemia (T-ALL) patients expressed CD1d and were good targets of Valpha24+ NKT cells. This cytotoxicity was increased in the presence of alpha-GalCer. Our results suggest that T-ALL is a good candidate for Valpha24+ NKT-cell-based immuno-cell therapy.
|
| Volume |
122(2)
|
| Pages |
231-9
|
| Published |
2003-7-1
|
| DOI |
10.1046/j.1365-2141.2003.04429.x
|
| PII |
4429
|
| PMID |
12846891
|
| MeSH |
Antigens, CD1 / analysis
Cytokines / immunology
Cytotoxicity Tests, Immunologic
Humans
Immunophenotyping
Immunotherapy, Adoptive / methods*
Killer Cells, Natural / immunology*
Leukemia-Lymphoma, Adult T-Cell / immunology*
Lymphocyte Activation
Receptors, Antigen, T-Cell, alpha-beta / immunology*
Tumor Cells, Cultured
|
| IF |
5.518
|
| Times Cited |
25
|
|
WOS Category
|
HEMATOLOGY
|
| Resource |
| Human and Animal Cells |
K562(RCB0027) |
