論文 - 詳細
| RRC ID | 41913 |
|---|---|
| 著者 | Nakai T, Kanamori T, Sando S, Aoyama Y. |
| タイトル | Remarkably size-regulated cell invasion by artificial viruses. Saccharide-dependent self-aggregation of glycoviruses and its consequences in glycoviral gene delivery. |
| ジャーナル | J Am Chem Soc |
| Abstract |
We here report a novel example of artificial glycoviral vectors constructed via number- and size-controlled gene (pCMVluc, 7040 bp) coating with micellar glycocluster nanoparticles (GNPs) of calix[4]resorcarene-based macrocyclic glycocluster amphiphiles having eight or five saccharide moieties with terminal alpha-glucose (alpha-Glc), beta-glucose (beta-Glc), or beta-galactose (beta-Gal) residues. The resulting glycoviruses are compactly packed (approximately 50 nm) and well charge-shielded (zeta approximately equal 0 mV), undergo saccharide-dependent (alpha-Glc > beta-Gal > beta-Glc) self-aggregation, and transfect cell (Hela and HepG2) cultures as triggered by the pinocytic form of endocytosis. The semilogarithmic linear size-activity correlation suggests that size-restricted pinocytosis (<100 nm) is effective only for monomeric viruses. The activities of oligomeric and otherwise poorly active beta-Gal-functionalized viruses toward hepatic HepG2 cells are approximately 10(2)-times higher than expected on the size basis, owing to the receptor-mediated specific pathway involving the asialoglycoprotein receptors on the hepatic cell surfaces. The scope and prospect of artificial glycoviruses are discussed. |
| 巻・号 | 125(28) |
| ページ | 8465-75 |
| 公開日 | 2003-7-16 |
| DOI | 10.1021/ja035636f |
| PMID | 12848552 |
| MeSH | Calixarenes* Cytomegalovirus / chemistry* Cytomegalovirus / genetics Genetic Vectors / chemistry* HeLa Cells Humans Luciferases / genetics Particle Size Phenols / chemistry Plasmids / administration & dosage Plasmids / chemistry Polysaccharides / chemistry* Promoter Regions, Genetic Transfection / methods |
| IF | 14.612 |
| 引用数 | 134 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) Hep G2(RCB1648) |