RRC ID 41913
Author Nakai T, Kanamori T, Sando S, Aoyama Y.
Title Remarkably size-regulated cell invasion by artificial viruses. Saccharide-dependent self-aggregation of glycoviruses and its consequences in glycoviral gene delivery.
Journal J Am Chem Soc
Abstract We here report a novel example of artificial glycoviral vectors constructed via number- and size-controlled gene (pCMVluc, 7040 bp) coating with micellar glycocluster nanoparticles (GNPs) of calix[4]resorcarene-based macrocyclic glycocluster amphiphiles having eight or five saccharide moieties with terminal alpha-glucose (alpha-Glc), beta-glucose (beta-Glc), or beta-galactose (beta-Gal) residues. The resulting glycoviruses are compactly packed (approximately 50 nm) and well charge-shielded (zeta approximately equal 0 mV), undergo saccharide-dependent (alpha-Glc > beta-Gal > beta-Glc) self-aggregation, and transfect cell (Hela and HepG2) cultures as triggered by the pinocytic form of endocytosis. The semilogarithmic linear size-activity correlation suggests that size-restricted pinocytosis (<100 nm) is effective only for monomeric viruses. The activities of oligomeric and otherwise poorly active beta-Gal-functionalized viruses toward hepatic HepG2 cells are approximately 10(2)-times higher than expected on the size basis, owing to the receptor-mediated specific pathway involving the asialoglycoprotein receptors on the hepatic cell surfaces. The scope and prospect of artificial glycoviruses are discussed.
Volume 125(28)
Pages 8465-75
Published 2003-7-16
DOI 10.1021/ja035636f
PMID 12848552
MeSH Calixarenes* Cytomegalovirus / chemistry* Cytomegalovirus / genetics Genetic Vectors / chemistry* HeLa Cells Humans Luciferases / genetics Particle Size Phenols / chemistry Plasmids / administration & dosage Plasmids / chemistry Polysaccharides / chemistry* Promoter Regions, Genetic Transfection / methods
IF 14.612
Times Cited 134
Human and Animal Cells HeLa(RCB0007) Hep G2