RRC ID |
41913
|
著者 |
Nakai T, Kanamori T, Sando S, Aoyama Y.
|
タイトル |
Remarkably size-regulated cell invasion by artificial viruses. Saccharide-dependent self-aggregation of glycoviruses and its consequences in glycoviral gene delivery.
|
ジャーナル |
J Am Chem Soc
|
Abstract |
We here report a novel example of artificial glycoviral vectors constructed via number- and size-controlled gene (pCMVluc, 7040 bp) coating with micellar glycocluster nanoparticles (GNPs) of calix[4]resorcarene-based macrocyclic glycocluster amphiphiles having eight or five saccharide moieties with terminal alpha-glucose (alpha-Glc), beta-glucose (beta-Glc), or beta-galactose (beta-Gal) residues. The resulting glycoviruses are compactly packed (approximately 50 nm) and well charge-shielded (zeta approximately equal 0 mV), undergo saccharide-dependent (alpha-Glc > beta-Gal > beta-Glc) self-aggregation, and transfect cell (Hela and HepG2) cultures as triggered by the pinocytic form of endocytosis. The semilogarithmic linear size-activity correlation suggests that size-restricted pinocytosis (<100 nm) is effective only for monomeric viruses. The activities of oligomeric and otherwise poorly active beta-Gal-functionalized viruses toward hepatic HepG2 cells are approximately 10(2)-times higher than expected on the size basis, owing to the receptor-mediated specific pathway involving the asialoglycoprotein receptors on the hepatic cell surfaces. The scope and prospect of artificial glycoviruses are discussed.
|
巻・号 |
125(28)
|
ページ |
8465-75
|
公開日 |
2003-7-16
|
DOI |
10.1021/ja035636f
|
PMID |
12848552
|
MeSH |
Calixarenes*
Cytomegalovirus / chemistry*
Cytomegalovirus / genetics
Genetic Vectors / chemistry*
HeLa Cells
Humans
Luciferases / genetics
Particle Size
Phenols / chemistry
Plasmids / administration & dosage
Plasmids / chemistry
Polysaccharides / chemistry*
Promoter Regions, Genetic
Transfection / methods
|
IF |
14.612
|
引用数 |
134
|
WOS 分野
|
CHEMISTRY, MULTIDISCIPLINARY
|
リソース情報 |
ヒト・動物細胞 |
HeLa(RCB0007)
Hep G2 |