RRC ID 41915
著者 Yang J, Ichikawa A, Tsuchiya T.
タイトル A novel function of connexin 32: marked enhancement of liver function in a hepatoma cell line.
ジャーナル Biochem Biophys Res Commun
Abstract Connexin 32 (Cx32) is the main gap junction protein in hepatocytes and plays an important role in the regulation of signal transfer and growth control in the liver by constructing gap junction channels and gap junctional intercellular communication (GJIC). In this study, the human Cx32 gene was transfected into a hepatoma cell line (HepG2) that showed aberrant expression of Cx32 and was deficient in GJIC. Cx32-transfected HepG2 not only expressed a higher level of Cx32 mRNA, but also showed increased GJIC compared with HepG2 and vector-transfected HepG2. Furthermore, the liver functions of ammonia removal and albumin secretion of HepG2 were markedly enhanced with Cx32 gene transfection. It may be expected to improve the cellular functions of the hepatoma cell line by Cx32 gene transfection and serve to develop an efficacious bioartificial liver.
巻・号 307(1)
ページ 80-5
公開日 2003-7-18
DOI 10.1016/s0006-291x(03)01117-3
PII S0006291X03011173
PMID 12849984
MeSH Albumins / metabolism Ammonia / metabolism Carcinoma, Hepatocellular / metabolism* Cell Communication / physiology Connexins / genetics Connexins / metabolism* Fluorescent Dyes / metabolism Gap Junctions / chemistry Gap Junctions / metabolism Humans Liver / metabolism* Liver Neoplasms / metabolism* Transfection Tumor Cells, Cultured
IF 2.985
引用数 36
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞