RRC ID 41922
Author Koshimura K, Tanaka J, Murakami Y, Kato Y.
Title Effect of high concentration of glucose on dopamine release from pheochromocytoma-12 cells.
Journal Metab. Clin. Exp.
Abstract To investigate the mechanism of the action of high concentration of glucose on transmitter release from neuronal cells, we examined the effect of high concentration of glucose on dopamine release from pheochromocytoma-12 (PC12) cells. When the cells were incubated with 9.0 or 13.5 mg/mL glucose (2- or 3-fold of the optimum glucose concentration for PC12 cells), dopamine release was increased in a dose-related manner. Glucose-induced increase in dopamine release was blunted by nicardipine, a Ca2+ channel blocker. Following addition of 13.5 mg/mL glucose, intracellular Ca2+ concentration was increased, which was eliminated by nicardipine. Administration of 9.0 or 13.5 mg/mL glucose induced membrane depolarization in a dose-related manner. Glucose-induced dopamine release was inhibited by pinacidil or diazoxide, adenosine triphosphate (ATP)-sensitive K+ channel (KATP channel) openers. These results suggest that a high concentration of glucose induced ATP production, which blocked the KATP channel to induce membrane depolarization, and increased intracellular Ca2+ concentration and dopamine release. When the cells were cultured with 9.0 or 13.5 mg/mL glucose for 7 days, high potassium chloride (KCl)-induced dopamine release and 45Ca2+ uptake were increased. These results suggest that long-term incubation with a high concentration of glucose increased the capacity of Ca2+ uptake to enhance depolarization-induced dopamine release from PC12 cells. These data taken together suggest that a high concentration of glucose induced activation of the Ca2+ channel to stimulate dopamine release from PC12 cells.
Volume 52(7)
Pages 922-6
Published 2003-7
DOI 10.1016/s0026-0495(03)00059-3
PII S0026049503000593
PMID 12870171
MeSH Adenosine Triphosphate / pharmacology Animals Calcium / metabolism Calcium Channel Blockers / pharmacology Calcium Channels / drug effects Calcium Channels / physiology Calcium Radioisotopes Diazoxide / pharmacology Dopamine / metabolism* Dose-Response Relationship, Drug Glucose / administration & dosage Glucose / pharmacology* Ion Channel Gating / drug effects Membrane Potentials / drug effects Mice Neurons / drug effects* Neurons / physiology Nicardipine / pharmacology PC12 Cells Pinacidil / pharmacology Potassium Channels / drug effects Potassium Channels / physiology Potassium Chloride / pharmacology Rats
IF 6.513
Times Cited 17
WOS Category ENDOCRINOLOGY & METABOLISM
Resource
Human and Animal Cells