RRC ID 41925
著者 Hirai H, Abe H, Tanaka K, Takatsu K, Sugamura K, Nakamura M, Nagata K.
タイトル Gene structure and functional properties of mouse CRTH2, a prostaglandin D2 receptor.
ジャーナル Biochem Biophys Res Commun
Abstract CRTH2, the second receptor for prostaglandin D(2) (PGD(2)), is thought to play a role in allergic inflammations through the induction of chemotactic migration and/or the activation of Th2, eosinophils, and basophils, in humans. We previously identified the mouse CRTH2 homolog of human CRTH2 and suggest that animal models would provide a clear understanding on the precise function of CRTH2 in allergic disorders. To this end we have confirmed that mouse CRTH2 is similar in gene structure to human CRTH2 and revealed that mouse CRTH2 is predominantly expressed in the eosinophils derived from IL-5-transgenic mice. Moreover, mouse CRTH2 harbors the ability to bind PGD(2) with high affinity and intracellular Ca(2+) mobilization in a Gi-dependent manner and chemotactic responses in several transfected cell lines. The results demonstrated here indicate that mouse CRTH2 is the functional ortholog of human CRTH2 and paves the way for future analysis of the in vivo functions of CRTH2.
巻・号 307(4)
ページ 797-802
公開日 2003-8-8
DOI 10.1016/s0006-291x(03)01266-x
PII S0006291X0301266X
PMID 12878180
MeSH Animals Calcium / metabolism Cell Line Cell Movement Gene Components Humans Mice Prostaglandin D2 / metabolism Prostaglandin D2 / pharmacology Receptors, Immunologic / genetics* Receptors, Immunologic / physiology* Receptors, Prostaglandin / genetics* Receptors, Prostaglandin / physiology*
IF 2.985
引用数 25
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 WEHI-1(RCB0035)