Reference - Detail
| RRC ID | 41932 |
|---|---|
| Author | Ito Y, Nogawa M. |
| Title | Preparation of a protein micro-array using a photo-reactive polymer for a cell-adhesion assay. |
| Journal | Biomaterials |
| Abstract |
A protein micro-array, called a "cell chip" was constructed by using a photo-reactive polymer for a cell-adhesion assay. Various amounts of albumin or fibronectin were covalently immobilized on a polystyrene dish using a micro-spotter with a dip pen. First, poly(acrylic acid) carrying azidophenyl groups was synthesized as the photo-reactive polymer. Secondly, the aqueous solution of a photo-reactive polymer (several nanoliters) was cast using the dip pen of the micro-spotter and dried in air. Subsequently, aqueous solutions of proteins were cast on the same place using the micro-spotter. After drying, the dish was irradiated with ultraviolet light. Finally, the immobilization was confirmed by staining with a dye. The immobilization was stable even after washing with Tween-20. The protein-immobilized area depended on the manipulation of the micro-spotter and the size of the dip pen. Subsequently, cell adhesion on the photo-immobilized protein micro-array was investigated. The adhesion behavior of cells depended on the kind of immobilized proteins and the kind of cells. The protein micro-array will be useful for cell diagnosis and for the selection of biomaterials to regulate cell behavior. |
| Volume | 24(18) |
| Pages | 3021-6 |
| Published | 2003-8-1 |
| DOI | 10.1016/s0142-9612(03)00134-0 |
| PII | S0142961203001340 |
| PMID | 12895574 |
| MeSH | Acrylic Resins Animals COS Cells Cell Adhesion / drug effects* Cell Adhesion / physiology Cell Culture Techniques / instrumentation* Cell Culture Techniques / methods Cell Line Chlorocebus aethiops Coated Materials, Biocompatible / pharmacology* Equipment Design Equipment Failure Analysis Fibronectins / pharmacology* Macrophages / cytology Macrophages / drug effects PC12 Cells Photochemistry / instrumentation Photochemistry / methods Polymers / radiation effects Protein Array Analysis / instrumentation* Protein Array Analysis / methods* Rats Serum Albumin, Bovine / pharmacology* |
| IF | 10.317 |
| Times Cited | 54 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) COS-7(RCB0539) PC-12(RCB0009) |