Reference - Detail
| RRC ID | 41962 |
|---|---|
| Author | Lee MH, Kwon TG, Park HS, Wozney JM, Ryoo HM. |
| Title | BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
BMP-2 stimulates the expression of three osteogenic master transcription factors: Runx2, Dlx5, and Osterix (Osx). However, the hierarchical regulatory relationships among them are not yet clearly understood. Osx was commonly stimulated in osteogenic and non-osteogenic cells in response to BMP-signaling, as Dlx5 was in our previous report. A cycloheximide experiment indicated that Osx expression by BMP-2 requires new protein synthesis. Even if Osx has been suggested as a downstream target of Runx2, the results of this study indicated that Osx expression was still induced by BMP-2 treatment in Runx2 null cells, but not induced by Runx2 overexpression in myogenic C2C12 cells. Instead, Osx expression by BMP-2 was completely abrogated by the antisense blocking of Dlx5. Depending upon the coincident expression pattern of Osx and Dlx5, and the blocking of Osx expression by the antisense Dlx5, BMP-2-induced Osx expression is mainly mediated not by Runx2, but by Dlx5. |
| Volume | 309(3) |
| Pages | 689-94 |
| Published | 2003-9-26 |
| DOI | 10.1016/j.bbrc.2003.08.058 |
| PII | S0006291X03016541 |
| PMID | 12963046 |
| MeSH | Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / pharmacology* Cell Differentiation Cell Line Core Binding Factor Alpha 1 Subunit Gene Expression Regulation Homeodomain Proteins / physiology* Mice Models, Biological Neoplasm Proteins* Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism* Rats Sp7 Transcription Factor Transcription Factors / biosynthesis* Transcription Factors / genetics Transcription Factors / physiology* Transforming Growth Factor beta* |
| IF | 2.985 |
| Times Cited | 289 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | ATDC5(RCB0565) MC3T3-E1(RCB1126) C2C12(RCB0987) ROS-17/2.8-5(RCB0462) |