| RRC ID |
41964
|
| 著者 |
Murakami Y, Shoji M, Hanazawa S, Tanaka S, Fujisawa S.
|
| タイトル |
Preventive effect of bis-eugenol, a eugenol ortho dimer, on lipopolysaccharide-stimulated nuclear factor kappa B activation and inflammatory cytokine expression in macrophages.
|
| ジャーナル |
Biochem Pharmacol
|
| Abstract |
Eugenol exhibits antioxidant and anti-inflammatory activities, but at higher concentrations acts as an oxidant and potent allergen. It was earlier shown that bis-eugenol synthesized by the oxidation of eugenol was less cytotoxic and more highly antioxidative than eugenol. But its anti-inflammatory mechanism remains yet unclear. Since nuclear factor-kappa B (NF-kappa B) is a key transcriptional factor in the expression of inflammatory cytokines, we examined whether eugenol and bis-eugenol are inhibitors of NF-kappa B activation. We observed that bis-eugenol, but not eugenol, clearly inhibited the degradation of inhibitory kappa B-alpha in RAW264.7 murine macrophages stimulated with lipopolysaccharide and, consequently, the transcriptional activity of the stimulated NF-kappa B in the cells. In addition, bis-eugenol actually inhibited LPS-stimulated expression of inflammatory cytokines at both gene and protein levels. These findings suggest that bis-eugenol acts as a potent inhibitor of NF-kappa B.
|
| 巻・号 |
66(6)
|
| ページ |
1061-6
|
| 公開日 |
2003-9-15
|
| DOI |
10.1016/s0006-2952(03)00419-2
|
| PII |
S0006295203004192
|
| PMID |
12963494
|
| MeSH |
Animals
Cytokines / metabolism*
Dimerization
Eugenol / pharmacology*
Inflammation
Lipopolysaccharides / pharmacology*
Macrophage Activation
Macrophages / drug effects*
Mice
NF-kappa B / metabolism*
|
| IF |
4.96
|
| 引用数 |
48
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
