| RRC ID |
41968
|
| Author |
Gruber R, Graninger W, Bobacz K, Watzek G, Erlacher L.
|
| Title |
BMP-6-induced osteogenic differentiation of mesenchymal cell lines is not modulated by sex steroids and resveratrol.
|
| Journal |
Cytokine
|
| Abstract |
Bone morphogenetic protein-6 (BMP-6) is a potent inducer of osteogenic differentiation and its expression is stimulated by 17beta-estradiol. The existence of a regulatory loop between sex steroids and BMP-6 is therefore reasonable to hypothesize. Here we determined whether the sex steroids 17beta-estradiol and dihydrotestosterone, and the phytoestrogen resveratrol can modulate BMP-6-induced alkaline phosphatase activity and osteocalcin expression. Mesenchymal cells of murine (osteoblastic MC3T3-E1 cells, preadipogenic ST2 cells, prechondrogenic ATDC5 cell) and human origin (osteosarcoma SaOS and HOS cells, primary bone marrow stromal cells) were cultured in the presence of recombinant BMP-6 under serum-free conditions. BMP-6 dose-, and time-dependently increased alkaline phosphatase activity in murine cell lines, but not in human cells. Osteocalcin expression was also increased upon stimulation with BMP-6. The presence of 17beta-estradiol, dihydrotestosterone, and resveratrol had no effect on BMP-6-induced alkaline phosphatase activity and osteocalcin expression. These data suggest that osteogenic differentiation in response to BMP-6 occurs independent of steroid hormones and resveratrol in mesenchymal cells that express basal receptor levels.
|
| Volume |
23(4-5)
|
| Pages |
133-7
|
| Published |
2003-8-21
|
| DOI |
10.1016/s1043-4666(03)00223-0
|
| PII |
S1043466603002230
|
| PMID |
12967649
|
| MeSH |
Alkaline Phosphatase / metabolism
Animals
Blotting, Northern
Bone Morphogenetic Protein 6
Bone Morphogenetic Proteins / pharmacology*
Cell Differentiation / drug effects*
Cell Differentiation / physiology
Cell Line
Cell Line, Tumor / drug effects
Dihydrotestosterone / pharmacology
Dose-Response Relationship, Drug
Estradiol / pharmacology
Gene Expression Regulation / drug effects
Gonadal Steroid Hormones / pharmacology*
Humans
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / metabolism
Mesoderm / cytology
Mesoderm / drug effects
Mesoderm / metabolism
Mice
Osteocalcin / genetics
Osteogenesis / drug effects*
Osteogenesis / physiology
RNA, Messenger / drug effects
RNA, Messenger / genetics
RNA, Messenger / metabolism
Resveratrol
Reverse Transcriptase Polymerase Chain Reaction
Stilbenes / pharmacology*
Time Factors
|
| IF |
2.952
|
| Times Cited |
19
|
|
WOS Category
|
IMMUNOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126)
ST2(RCB0224) |
