Reference - Detail
| RRC ID | 41988 |
|---|---|
| Author | Juliet PA, Hayashi T, Iguchi A, Ignarro LJ. |
| Title | Concomitant production of nitric oxide and superoxide in human macrophages. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Many harmful effects of nitric oxide are caused by the reaction of NO with superoxide anion. The present study was carried out to find out the concomitant production of superoxide and to investigate a suitable inhibitor of NO, which is produced by iNOS. THP-1 cells were differentiated into macrophages by PMA and cytokine. Addition of L-NAME showed decrement in superoxide production. Addition of apocynin, aminoguanidine or ONO 1714 brought about a significant reduction in superoxide production. The expressions of p67 and p47(phox) were reduced by the addition of apocynin, aminoguanidine or ONO 1714 whereas xanthine oxidase and cyclooxygenase did not have a major role in superoxide production. The results of the present study show that iNOS and NADPH oxidase play an important role in superoxide release. It suggests that addition of iNOS inhibitor together with apocynin may be more effective in case of therapeutic application in disease conditions like atherosclerosis. |
| Volume | 310(2) |
| Pages | 367-70 |
| Published | 2003-10-17 |
| DOI | 10.1016/j.bbrc.2003.08.133 |
| PII | S0006291X03017066 |
| PMID | 14521919 |
| MeSH | Acetophenones / pharmacology Allopurinol / pharmacology Amidines / pharmacology Aspirin / pharmacology Cell Line Cyclooxygenase Inhibitors / pharmacology Enzyme Inhibitors / pharmacology Guanidines / pharmacology Heterocyclic Compounds, 2-Ring / pharmacology Humans Macrophages / drug effects Macrophages / enzymology Macrophages / metabolism* NADPH Oxidases / metabolism NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide / biosynthesis* Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II Superoxides / metabolism* Xanthine Oxidase / metabolism |
| IF | 2.985 |
| Times Cited | 30 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | THP-1(RCB1189) |