RRC ID 41998
Author Akalal DB, Bottenstein JE, Lee SH, Han JH, Chang DJ, Kaang BK, Nagle GT.
Title Aplysia mollusk-derived growth factor is a mitogen with adenosine deaminase activity and is expressed in the developing central nervous system.
Journal Brain Res Mol Brain Res
Abstract Mollusk-derived growth factor (MDGF), the first growth factor to be characterized in Aplysia, was purified and characterized and has both adenosine deaminase activity and stimulates cell proliferation in vitro. MDGF is structurally related to a new subfamily of adenosine deaminase-related growth factors that require enzymatic activity to stimulate cell proliferation, a unique property of known growth factors. We examined the expression of MDGF protein in the CNS since MDGF mRNA increased in the developing CNS, and recent data suggest that inosine is involved in neuronal reorganization and restoration of essential circuitry after CNS injury. MDGF levels transiently increased during embryonic and post-metamorphic development and in the developing CNS, but was undetectable in adult CNS. No effects on morphology or neurite extension of adult Aplysia neurons were observed.
Volume 117(2)
Pages 228-36
Published 2003-10-7
DOI 10.1016/s0169-328x(03)00287-0
PII S0169328X03002870
PMID 14559158
MeSH Adenosine Deaminase / metabolism* Animals Aplysia Blotting, Western Cell Count Cell Division / drug effects Cell Division / physiology Cell Line Central Nervous System / growth & development Central Nervous System / metabolism* Drosophila Gene Expression Regulation, Developmental* Growth Substances / chemistry Growth Substances / metabolism* Growth Substances / pharmacology In Vitro Techniques Indoles / metabolism Inosine / metabolism Intercellular Signaling Peptides and Proteins / chemistry Intercellular Signaling Peptides and Proteins / metabolism* Intercellular Signaling Peptides and Proteins / pharmacology Mitogens / metabolism Molecular Sequence Data Ovum / growth & development Ovum / metabolism Time Factors
Times Cited 14
Human and Animal Cells