| RRC ID |
42005
|
| 著者 |
Tojo Y, Bandoh S, Fujita J, Kubo A, Ishii T, Fukunaga Y, Ueda Y, Yang Y, Wu F, Huang CL, Yokomise H, Ishida T.
|
| タイトル |
Aberrant messenger RNA splicing of the cytokeratin 8 in lung cancer.
|
| ジャーナル |
Lung Cancer
|
| Abstract |
Cytokeratin 8 (CK8) is one of the cytoskeletal components and shows caspase-mediated degradation when cells undergo apoptosis. We previously reported that CK8 is highly expressed in non-small cell lung cancer (NSCLC) cell lines and increasing values of serum CK8 are significantly associated with tumor progression in patients with NSCLC. In this investigation, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis in lung cancer cell lines, revealed a shorter PCR product, which differed from the wild-type product of CK8. The nucleotide sequence of the shorter PCR products and genomic DNA for CK8 demonstrated that the shorter product was an aberrantly spliced form of CK8 (AS-CK8) which lacked a caspases cleavage site within the linker lesion in exon 5. The putative protein products predicted by the mRNA of AS-CK8 were demonstrated by Western blotting with monoclonal antibodies for CK8. In addition, AS-CK8 mRNA and its protein products were highly expressed in NSCLC cell lines compared with small-cell lung cancer (SCLC) cell lines. Tissue samples obtained from NSCLC patients also expressed mRNA of AS-CK8. In conclusion, we identified aberrantly spliced CK8 (AS-CK8) which lacked a caspases cleavage site in lung cancer cell lines and primary tumors of NSCLC. AS-CK8 was preferentially expressed in NSCLC, rather than SCLC. These findings lead to speculation that cancer cells expressing AS-CK8 may have a resistance to apoptosis and may perturb keratin network formation.
|
| 巻・号 |
42(2)
|
| ページ |
153-61
|
| 公開日 |
2003-11-1
|
| DOI |
10.1016/s0169-5002(03)00288-5
|
| PII |
S0169500203002885
|
| PMID |
14568682
|
| MeSH |
Apoptosis
Base Sequence
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / pathology
Carcinoma, Small Cell / genetics*
Carcinoma, Small Cell / pathology
Humans
Keratins / biosynthesis
Keratins / genetics*
Lung Neoplasms / genetics*
Lung Neoplasms / pathology
Molecular Sequence Data
RNA Splice Sites*
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
|
| IF |
4.702
|
| 引用数 |
8
|
|
WOS 分野
|
RESPIRATORY SYSTEM
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
LU-165(RCB1184)
LC-1/sq(RCB0445) |
