論文 - 詳細
| RRC ID | 42005 |
|---|---|
| 著者 | Tojo Y, Bandoh S, Fujita J, Kubo A, Ishii T, Fukunaga Y, Ueda Y, Yang Y, Wu F, Huang CL, Yokomise H, Ishida T. |
| タイトル | Aberrant messenger RNA splicing of the cytokeratin 8 in lung cancer. |
| ジャーナル | Lung Cancer |
| Abstract |
Cytokeratin 8 (CK8) is one of the cytoskeletal components and shows caspase-mediated degradation when cells undergo apoptosis. We previously reported that CK8 is highly expressed in non-small cell lung cancer (NSCLC) cell lines and increasing values of serum CK8 are significantly associated with tumor progression in patients with NSCLC. In this investigation, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis in lung cancer cell lines, revealed a shorter PCR product, which differed from the wild-type product of CK8. The nucleotide sequence of the shorter PCR products and genomic DNA for CK8 demonstrated that the shorter product was an aberrantly spliced form of CK8 (AS-CK8) which lacked a caspases cleavage site within the linker lesion in exon 5. The putative protein products predicted by the mRNA of AS-CK8 were demonstrated by Western blotting with monoclonal antibodies for CK8. In addition, AS-CK8 mRNA and its protein products were highly expressed in NSCLC cell lines compared with small-cell lung cancer (SCLC) cell lines. Tissue samples obtained from NSCLC patients also expressed mRNA of AS-CK8. In conclusion, we identified aberrantly spliced CK8 (AS-CK8) which lacked a caspases cleavage site in lung cancer cell lines and primary tumors of NSCLC. AS-CK8 was preferentially expressed in NSCLC, rather than SCLC. These findings lead to speculation that cancer cells expressing AS-CK8 may have a resistance to apoptosis and may perturb keratin network formation. |
| 巻・号 | 42(2) |
| ページ | 153-61 |
| 公開日 | 2003-11-1 |
| DOI | 10.1016/s0169-5002(03)00288-5 |
| PII | S0169500203002885 |
| PMID | 14568682 |
| MeSH | Apoptosis Base Sequence Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Non-Small-Cell Lung / pathology Carcinoma, Small Cell / genetics* Carcinoma, Small Cell / pathology Humans Keratins / biosynthesis Keratins / genetics* Lung Neoplasms / genetics* Lung Neoplasms / pathology Molecular Sequence Data RNA Splice Sites* RNA, Messenger / biosynthesis Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured |
| IF | 4.702 |
| 引用数 | 8 |
| WOS 分野 | RESPIRATORY SYSTEM ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | LC-1/sq(RCB0445) LU-165(RCB1184) |