論文 - 詳細
| RRC ID | 42009 |
|---|---|
| 著者 | Tanabe M, Kurita-Taniguchi M, Takeuchi K, Takeda M, Ayata M, Ogura H, Matsumoto M, Seya T. |
| タイトル | Mechanism of up-regulation of human Toll-like receptor 3 secondary to infection of measles virus-attenuated strains. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
PolyI:C, a synthetic double-stranded (ds)RNA, and viruses act on cells to induce IFN-beta which is a key molecule for anti-viral response. Although dsRNA is a virus-specific signature and a ligand for human Toll-like receptor 3 (TLR3), largely uncharacterized multiple pathways associate virus-mediated IFN-beta induction. Here, we demonstrated that laboratory-adapted but not wild-type strains of measles virus (MV) up-regulated TLR3 expression both in dendritic cells and epithelial cell line A549. The kinetics experiments with the laboratory MV strain revealed that TLR3 was induced late compared to IFN-beta and required new protein synthesis. Furthermore, neutralizing antibodies against IFN-beta or IFNAR (Interferon-alpha/beta receptor) suppressed MV-induced TLR3 induction, indicating that type I IFN, IFN-alpha/beta, is critical for MV-mediated TLR3 induction. Yet, a recently identified virus-inducible IFN, the IFN-lambda, did not contribute to TLR3 expression. A virus-responsive element that up-regulates TLR3 was identified in the TLR3-promoter region by reporter gene experiments. The ISRE, a recently reported site for IFN-beta induction, but not STAT binding site, located around -30bp of TLR3 promoter responded to MV to induce TLR3 expression. This further indicates the importance of type I IFN for TLR3 up-regulation in the case of viral infection. In HeLa and MRC5 cells, augmented production of IFN-beta was observed in response to dsRNA when TLR3 had been induced beforehand. Thus, the MV-induced expression of TLR3 may reflect amplified IFN production that plays a part in host defense to viral infection. |
| 巻・号 | 311(1) |
| ページ | 39-48 |
| 公開日 | 2003-11-7 |
| DOI | 10.1016/j.bbrc.2003.09.159 |
| PII | S0006291X03019600 |
| PMID | 14575692 |
| MeSH | Cell Line Fibroblasts / immunology Fibroblasts / metabolism Fibroblasts / virology Humans Interferon-beta / immunology* Interferon-beta / metabolism* Measles / immunology Measles / metabolism* Measles virus / classification Measles virus / immunology Measles virus / pathogenicity* Membrane Glycoproteins / classification Membrane Glycoproteins / immunology Membrane Glycoproteins / metabolism* Receptors, Cell Surface / classification Receptors, Cell Surface / immunology Receptors, Cell Surface / metabolism* Respiratory Mucosa / immunology Respiratory Mucosa / metabolism Respiratory Mucosa / virology Toll-Like Receptor 3 Toll-Like Receptors Up-Regulation / physiology* Vaccines, Attenuated |
| IF | 2.985 |
| 引用数 | 81 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) MRC-5 |