| RRC ID |
42015
|
| Author |
Kokura S, Yoshida N, Ueda M, Imamoto E, Ishikawa T, Takagi T, Naito Y, Okanoue T, Yoshikawa T.
|
| Title |
Hyperthermia enhances tumor necrosis factor alpha-induced apoptosis of a human gastric cancer cell line.
|
| Journal |
Cancer Lett
|
| Abstract |
We have investigated the effects of hyperthermia on the apoptosis induced by tumor necrosis factor alpha (TNF-alpha). Confluent monolayers of human gastric cancer cell line MKN45 were either treated or untreated with hyperthermia for 1 h. The cells were subsequently stimulated with TNF-alpha. A 24-h incubation with TNF-alpha did not affect cell viabilities; however, pretreatment with hyperthermia significantly enhanced the level of apoptosis induced by TNF-alpha. Pretreating MKN45 cells with hyperthermia (42.0 degrees C) significantly inhibited the TNF-alpha-induced increase in the binding activity of NF-kappaB to DNA. This study suggests that hyperthermia can inhibit the TNF-alpha-induced NF-kappaB activation and that hyperthermia renders human gastric cancer cells susceptible to the TNF-alpha-induced apoptosis, possibly via inhibition of the NF-kappaB pathway.
|
| Volume |
201(1)
|
| Pages |
89-96
|
| Published |
2003-11-10
|
| DOI |
10.1016/s0304-3835(03)00463-4
|
| PII |
S0304383503004634
|
| PMID |
14580690
|
| MeSH |
Apoptosis / drug effects
Apoptosis / physiology*
Cell Line, Tumor
Cell Survival
DNA Fragmentation
Fever*
Humans
NF-kappa B / drug effects
NF-kappa B / metabolism
Stomach Neoplasms / pathology*
Tumor Necrosis Factor-alpha / pharmacology
|
| IF |
7.36
|
| Times Cited |
17
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
MKN45(RCB1001) |
