RRC ID 42033
Author Bhattacharyya RS, Stern PH.
Title IGF-I and MAP kinase involvement in the stimulatory effects of LNCaP prostate cancer cell conditioned media on cell proliferation and protein synthesis in MC3T3-E1 osteoblastic cells.
Journal J Cell Biochem
Abstract Bone metastases from prostate cancer cause abnormal new bone formation, however, the factors involved and the pathways leading to the response are incompletely defined. We investigated the mechanisms of osteoblast stimulatory effects of LNCaP prostate carcinoma cell conditioned media (CM). MC3T3-E1 osteoblastic cells were cultured with CM from confluent LNCaP cells. LNCaP CM stimulated MAP kinase, cell proliferation (3H-thymidine incorporation), and protein synthesis (14C-proline incorporation) in the MC3T3-E1 cells. The increases in cell proliferation and protein synthesis were prevented by inhibition of the MAP kinase pathway. IGF-I mimicked the effects of the CM on the MC3T3-E1 cells and inhibition of IGF-I action decreased the LNCaP CM stimulation of 3H-thymidine and 14C-proline incorporation and MAP kinase activity. The findings indicate that IGF-I is an important factor for the stimulatory effects of LNCaP cell CM on cell proliferation and protein synthesis in osteoblastic cells, and that MAP kinase is a component of the signaling pathway for these effects.
Volume 90(5)
Pages 925-37
Published 2003-12-1
DOI 10.1002/jcb.10726
PMID 14624452
MeSH Animals Cell Division / drug effects Culture Media, Conditioned Enzyme Inhibitors / pharmacology Humans Insulin-Like Growth Factor I / antagonists & inhibitors Insulin-Like Growth Factor I / metabolism* Male Mice Mitogen-Activated Protein Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / metabolism* Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism* Proline / metabolism Prostatic Neoplasms / metabolism* Signal Transduction* Thymidine / metabolism Tumor Cells, Cultured
IF 3.448
Times Cited 8
Human and Animal Cells