| RRC ID |
42035
|
| Author |
Kato K, Umezawa K, Funeriu DP, Miyake M, Miyake J, Nagamune T.
|
| Title |
Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface.
|
| Journal |
Biotechniques
|
| Abstract |
Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology.
|
| Volume |
35(5)
|
| Pages |
1014-8, 1020-1
|
| Published |
2003-11-1
|
| DOI |
10.2144/03355rr01
|
| PMID |
14628675
|
| MeSH |
Animals
Biocompatible Materials / chemical synthesis*
Biocompatible Materials / chemistry
Biosensing Techniques / instrumentation
Biosensing Techniques / methods
Cell Adhesion / physiology*
Cell Culture Techniques / methods*
Cell Division / physiology*
Cell Survival / physiology
Cells, Immobilized / physiology*
Ether / chemistry*
Humans
Jurkat Cells
K562 Cells
Membranes, Artificial
Mice
NIH 3T3 Cells
Polyethylene Glycols / chemistry*
Surface Properties
|
| IF |
1.541
|
| Times Cited |
61
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
NIH/3T3 |
