Reference - Detail
| RRC ID | 42035 |
|---|---|
| Author | Kato K, Umezawa K, Funeriu DP, Miyake M, Miyake J, Nagamune T. |
| Title | Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface. |
| Journal | Biotechniques |
| Abstract |
Microarrays of living cells are an emerging tool in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology. |
| Volume | 35(5) |
| Pages | 1014-8, 1020-1 |
| Published | 2003-11-1 |
| DOI | 10.2144/03355rr01 |
| PMID | 14628675 |
| MeSH | Animals Biocompatible Materials / chemical synthesis* Biocompatible Materials / chemistry Biosensing Techniques / instrumentation Biosensing Techniques / methods Cell Adhesion / physiology* Cell Culture Techniques / methods* Cell Division / physiology* Cell Survival / physiology Cells, Immobilized / physiology* Ether / chemistry* Humans Jurkat Cells K562 Cells Membranes, Artificial Mice NIH 3T3 Cells Polyethylene Glycols / chemistry* Surface Properties |
| IF | 1.541 |
| Times Cited | 61 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 15 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NIH/3T3 |