| RRC ID |
42050
|
| 著者 |
King DW, Steinmetz R, Wagoner HA, Hannon TS, Chen LY, Eugster EA, Pescovitz OH.
|
| タイトル |
Differential expression of GRK isoforms in nonmalignant and malignant human granulosa cells.
|
| ジャーナル |
Endocrine
|
| Abstract |
Granulosa cell tumors are serious ovarian neoplasms that can occur in women of all ages. While there have been numerous attempts to understand the cause of these malignancies, the pathogenesis of granulosa cell tumors (GCTs) still remains largely unknown. G-protein coupled receptor kinases (GRKs) are important regulators of signal transduction through the process of receptor desensitization and internalization. Receptors that are regulated by GRKs are members of the large family of seven-transmembrane receptors and include the follicle stimulating hormone receptor (FSHR). In granulosa cells, the FSH signaling system is responsible for cell proliferation, differentiation, and steroidogenesis. In the studies presented, we examined GRK mRNA and protein expression in nonmalignant human granulosa cells, in KGN cells, a human GCT cell line, and in a panel of human GCT samples. The KGN tumor cells express significantly less GRK4 alpha/beta protein and higher levels of GRK2 and GRK4 gamma/delta protein as compared to nonmalignant human granulosa cells. In human GCT samples, GRK4 alpha/beta protein was detected in 3 of the 13 tumor samples, whereas gamma/delta proteins expression was detected in all samples. These findings suggest that GRK protein expression is altered in GCTs and may be involved in the pathogenesis of these tumors.
|
| 巻・号 |
22(2)
|
| ページ |
135-42
|
| 公開日 |
2003-11-1
|
| DOI |
10.1385/ENDO:22:2:135
|
| PII |
ENDO:22:2:135
|
| PMID |
14665717
|
| MeSH |
Blotting, Western
Cell Line, Tumor
Female
Gene Expression Regulation
Gene Expression Regulation, Neoplastic
Granulosa Cell Tumor / chemistry*
Humans
Ovarian Neoplasms / chemistry*
Protein Isoforms
Protein Kinases / analysis*
RNA, Messenger / analysis
Receptors, FSH / analysis*
Receptors, G-Protein-Coupled / analysis*
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
3.235
|
| 引用数 |
31
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
KGN(RCB1154) |
