| RRC ID |
42066
|
| 著者 |
Ito A, Matsuoka F, Honda H, Kobayashi T.
|
| タイトル |
Heat shock protein 70 gene therapy combined with hyperthermia using magnetic nanoparticles.
|
| ジャーナル |
Cancer Gene Ther
|
| Abstract |
Heat shock proteins (HSPs) are recognized as significant participants in immune reactions. We previously reported that expression of HSP70 in response to hyperthermia, produced using our original magnetite cationic liposomes (MCLs), induces antitumor immunity. In the present study, we examine whether the antitumor immunity induced by hyperthermia is enhanced by hsp70 gene transfer. A human hsp70 gene mediated by cationic liposomes was injected into a B16 melanoma nodule in C57BL/6 mice in situ. At 24 hours after the injection of the hsp70 gene, MCLs were injected into melanoma nodules in C57BL/6 mice, which were subjected to an alternating magnetic field for 30 minutes. The temperature at the tumor reached 43 degrees C and was maintained by controlling the magnetic field intensity. The combined treatment strongly arrested tumor growth over a 30-day period, and complete regression of tumors was observed in 30% (3/10) of mice. Systemic antitumor immunity was induced in the cured mice. This study demonstrates that this novel therapeutic strategy combining the use of hsp70 gene therapy and hyperthermia using MCLs may be applicable to patients with advanced malignancies.
|
| 巻・号 |
10(12)
|
| ページ |
918-25
|
| 公開日 |
2003-12-1
|
| DOI |
10.1038/sj.cgt.7700648
|
| PII |
7700648
|
| PMID |
14712318
|
| MeSH |
Animals
Cell Division
Combined Modality Therapy
Electromagnetic Fields
Ferrosoferric Oxide
Gene Transfer Techniques
Genetic Therapy*
HSP70 Heat-Shock Proteins / genetics*
Hyperthermia, Induced* / veterinary
Iron
Liposomes
Melanoma / genetics*
Melanoma / therapy*
Melanoma / veterinary
Mice
Mice, Inbred C57BL
Nanotechnology
Neoplasms, Experimental
Oxides
Particle Size
Skin Neoplasms / genetics*
Skin Neoplasms / therapy*
Skin Neoplasms / veterinary
Treatment Outcome
|
| IF |
4.534
|
| 引用数 |
56
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma(RCB1283) |
