論文 - 詳細
| RRC ID | 42072 |
|---|---|
| 著者 | Tazume K, Hagihara M, Gansuvd B, Higuchi A, Ueda Y, Hirabayashi K, Hojo M, Tanabe A, Okamoto A, Kato S, Hotta T. |
| タイトル | Induction of cytomegalovirus-specific CD4+ cytotoxic T lymphocytes from seropositive or negative healthy subjects or stem cell transplant recipients. |
| ジャーナル | Exp Hematol |
| Abstract |
OBJECTIVE:We generated cytomegalovirus (CMV)-specific cytotoxic T lymphocytes (CTL) in vitro using dendritic cells (DC) pulsed with crude CMV antigens (Ag). PATIENTS AND METHODS:Mononuclear cells from healthy CMV-seropositive or seronegative volunteers and from stem cell transplant (SCT) recipients were cultured with CD14(+) monocyte-derived DC prepulsed with CMV Ag and then matured in vitro with lipopolysaccharide and tumor necrosis factor-alpha. After proliferation, cells were checked for phenotype (CD4/CD8), while killing activity was measured by 51Cr-release assay. RESULTS:CD4(+) T cells, the main proliferating cells from both seropositive and seronegative individuals, killed autologous Ag-pulsed DC but not vehicle-pulsed autologous DC or CMV-pulsed allogeneic DC. Similar CTL induction was accomplished from SCT recipients. Significant killing of autologous CMV-infected fibroblasts required 16-hour incubation as opposed to the standard 4-hour incubation, which was prevented by either a perforin inhibitor or anti-Fas ligand monoclonal antibody. CTL enhanced surface HLA-DR expression of CMV-infected fibroblasts, and their activity was neutralized by anti-HLA-DR monoclonal antibody. CONCLUSION:CMV-specific CD4(+) CTL were inducible with or without antiviral humoral immunity, even from immunosuppressed SCT recipients. These CTL showed perforin- and Fas/Fas ligand-mediated cytotoxicity after long-term (16-hour) contact with CMV-infected targets. |
| 巻・号 | 32(1) |
| ページ | 95-103 |
| 公開日 | 2004-1-1 |
| DOI | 10.1016/j.exphem.2003.10.008 |
| PII | S0301472X03003497 |
| PMID | 14725906 |
| MeSH | Antigens, Viral / immunology CD4-Positive T-Lymphocytes / immunology* Cytomegalovirus / immunology* Cytomegalovirus Infections / immunology* Cytotoxicity, Immunologic Dendritic Cells / physiology HLA-DR Antigens / analysis Hematopoietic Stem Cell Transplantation* Humans T-Lymphocytes, Cytotoxic / immunology* |
| IF | 2.82 |
| 引用数 | 9 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL HEMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5 K562 |