RRC ID 42073
著者 Ogura Y, Suruga K, Mochizuki H, Yamamoto T, Takase S, Goda T.
タイトル Postnatal changes in gene expression of retinal dehydrogenase and retinoid receptors in liver of rats.
ジャーナル Life Sci
Abstract Retinoic acid (RA) plays important roles in cellular differentiation and proliferation in various tissues including the liver. To explore a possible role of RA in the postnatal development of hepatic function, we analyzed RA-generation enzyme activity and the RA-related hepatic gene expressions in the suckling and weaning rats. At 5 days after birth, retinal dehydrogenase (RALDH) activity in the liver was relatively high. Its activity decreased by 70% until day 17, and then it gradually increased to a high level by the completion of weaning period. Northern blot analysis showed that RALDH2 mRNA levels decreased in the suckling period, whereas RALDH1 mRNA levels increased in the weaning period. Retinoid X receptor alpha (RXRalpha) mRNA levels increased in the suckling period and attained to a higher level at 17 days after birth. Retinoic acid receptor alpha (RARalpha) mRNA level showed only a slight and temporary increase on day 13. The mRNA levels of hepatocyte nuclear factors (HNF-4 and HNF-1alpha) exhibited parallel increases around suckling-weaning period, and the transcript levels of albumin, a typical target gene of the hepatocyte nuclear factors, increased during the suckling-weaning transition period. Electrophoretic mobility shift assay using a putative nuclear receptor-binding element on rat HNF-1 alpha gene revealed that HNF-4 homodimer, but not RXRalpha homodimer, bound to this element. These results suggest that postnatal expressions of hepatocyte-specific genes might be up-regulated by retinoid receptors, which may be related with the alterations of RALDH expression during postnatal development in the liver.
巻・号 74(12)
ページ 1519-28
公開日 2004-2-6
DOI 10.1016/j.lfs.2003.08.020
PII S0024320503010415
PMID 14729401
MeSH Aldehyde Oxidoreductases / genetics Aldehyde Oxidoreductases / metabolism* Animals Animals, Newborn Animals, Suckling DNA-Binding Proteins* Dimerization Female Gene Expression Regulation* Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Hepatocyte Nuclear Factor 4 Isoenzymes / genetics Isoenzymes / metabolism* Liver / metabolism* Nuclear Proteins* Phosphoproteins / genetics Phosphoproteins / metabolism Protein Binding RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Receptors, Retinoic Acid / genetics Receptors, Retinoic Acid / metabolism* Retinal Dehydrogenase Transcription Factors / genetics Transcription Factors / metabolism Tretinoin / metabolism Weaning
IF 3.647
引用数 8
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞