| RRC ID |
42074
|
| 著者 |
Hara T, Ishii T, Fujishiro M, Masuda M, Ito T, Nakajima J, Inoue T, Matsuse T.
|
| タイトル |
Glutathione S-transferase P1 has protective effects on cell viability against camptothecin.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
Glutathione S-transferase P1 (GSTP1) is one of the important xenobiotic-metabolizing enzymes. It was reported that GSTP1 was overexpressed in malignant tissues, and its expression level was associated with resistance to chemotherapeutics. We carried out transfection of GSTP1 sense and antisense vectors to examine effects of GSTP1 on cell cycle arrest and apoptosis induced by camptothecin in HeLa cells. Transfection of GSTP1 antisense vector induced apoptosis. Camptothecin-induced S- or G2/M arrest was intensified by transfection of GSTP1 antisense vector, and subsequent apoptosis was attenuated by transfection of GSTP1 sense vector. These results suggest that GSTP1 has protective effects against camptothecin-induced cytotoxicity.
|
| 巻・号 |
203(2)
|
| ページ |
199-207
|
| 公開日 |
2004-1-20
|
| DOI |
10.1016/j.canlet.2003.09.005
|
| PII |
S0304383503005895
|
| PMID |
14732228
|
| MeSH |
Antineoplastic Agents, Phytogenic / pharmacology
Apoptosis
Camptothecin / pharmacology*
Cell Cycle
Cell Division
Cell Separation
Cell Survival / drug effects
DNA / metabolism
Dose-Response Relationship, Drug
Flow Cytometry
G2 Phase
Genetic Vectors
Glutathione S-Transferase pi
Glutathione Transferase / physiology*
HeLa Cells
Humans
Immunoblotting
Magnetics
Mitosis
Oligonucleotides, Antisense / pharmacology
S Phase
Time Factors
Transfection
Xenobiotics / pharmacology
|
| IF |
7.36
|
| 引用数 |
9
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
