論文 - 詳細
| RRC ID | 42081 |
|---|---|
| 著者 | Ouchi T, Yamabe E, Hara K, Hirai M, Ohya Y. |
| タイトル | Design of attachment type of drug delivery system by complex formation of avidin with biotinyl drug model and biotinyl saccharide. |
| ジャーナル | J Control Release |
| Abstract |
Recent studies have focused on the active targeting of drug delivery by combining a homing device and antitumor drug. For this purpose, synthesis of a well-designed vehicle (such as polymer/drug conjugates or nanoparticles) carrying a drug and a homing device requires many steps. We propose a new type of drug delivery system (DDS) by formation of a complex containing avidin (Av) plus biotinyl drug with a biotinyl homing device, which easily accommodates the combination of various drugs and homing devices. The targetable drug complex can be prepared by selecting an appropriate biotinyl drug derivative and a biotinyl homing device and mixing them with avidin. Fluorescent dye with 5-(and-6)-carboxytetramethylrhodamine (TAMRA) was used as a drug model, and galactose (Gal) recognized by liver parenchymal cells was used as a homing device. TAMRA and galactose were attached to biotin (Bio) through a triethyleneglycol (TEG) spacer group to give Bio-TEG-TAMRA conjugate and Bio-TEG-Gal conjugate, respectively. Confocal laser scanning microscopic studies suggest that the complexes prepared by mixing Bio-TEG-Gal conjugate and fluorescein isothiocyanate (FITC)-labeled Av (feed molar ratio 4:1), and mixing Bio-TEG-Gal conjugate, Bio-TEG-TAMRA conjugate and FITC-labeled Av are internalized into the hepatoma cells through a receptor-mediated endocytosis mechanism. |
| 巻・号 | 94(2-3) |
| ページ | 281-91 |
| 公開日 | 2004-2-10 |
| DOI | 10.1016/j.jconrel.2003.09.020 |
| PII | S0168365903004838 |
| PMID | 14744480 |
| MeSH | Avidin / chemical synthesis* Avidin / metabolism Biotinylation / methods* Cell Line, Tumor Drug Delivery Systems / methods* Galactose / chemical synthesis* Galactose / metabolism Humans Rhodamines / chemical synthesis* Rhodamines / metabolism |
| IF | 7.727 |
| 引用数 | 20 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |