RRC ID |
42081
|
著者 |
Ouchi T, Yamabe E, Hara K, Hirai M, Ohya Y.
|
タイトル |
Design of attachment type of drug delivery system by complex formation of avidin with biotinyl drug model and biotinyl saccharide.
|
ジャーナル |
J Control Release
|
Abstract |
Recent studies have focused on the active targeting of drug delivery by combining a homing device and antitumor drug. For this purpose, synthesis of a well-designed vehicle (such as polymer/drug conjugates or nanoparticles) carrying a drug and a homing device requires many steps. We propose a new type of drug delivery system (DDS) by formation of a complex containing avidin (Av) plus biotinyl drug with a biotinyl homing device, which easily accommodates the combination of various drugs and homing devices. The targetable drug complex can be prepared by selecting an appropriate biotinyl drug derivative and a biotinyl homing device and mixing them with avidin. Fluorescent dye with 5-(and-6)-carboxytetramethylrhodamine (TAMRA) was used as a drug model, and galactose (Gal) recognized by liver parenchymal cells was used as a homing device. TAMRA and galactose were attached to biotin (Bio) through a triethyleneglycol (TEG) spacer group to give Bio-TEG-TAMRA conjugate and Bio-TEG-Gal conjugate, respectively. Confocal laser scanning microscopic studies suggest that the complexes prepared by mixing Bio-TEG-Gal conjugate and fluorescein isothiocyanate (FITC)-labeled Av (feed molar ratio 4:1), and mixing Bio-TEG-Gal conjugate, Bio-TEG-TAMRA conjugate and FITC-labeled Av are internalized into the hepatoma cells through a receptor-mediated endocytosis mechanism.
|
巻・号 |
94(2-3)
|
ページ |
281-91
|
公開日 |
2004-2-10
|
DOI |
10.1016/j.jconrel.2003.09.020
|
PII |
S0168365903004838
|
PMID |
14744480
|
MeSH |
Avidin / chemical synthesis*
Avidin / metabolism
Biotinylation / methods*
Cell Line, Tumor
Drug Delivery Systems / methods*
Galactose / chemical synthesis*
Galactose / metabolism
Humans
Rhodamines / chemical synthesis*
Rhodamines / metabolism
|
IF |
7.727
|
引用数 |
20
|
WOS 分野
|
CHEMISTRY, MULTIDISCIPLINARY
PHARMACOLOGY & PHARMACY
|
リソース情報 |
ヒト・動物細胞 |
Hep G2 |