論文 - 詳細
RRC ID | 421 |
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著者 | Broday L, Kolotuev I, Didier C, Bhoumik A, Podbilewicz B, Ronai Z. |
タイトル | The LIM domain protein UNC-95 is required for the assembly of muscle attachment structures and is regulated by the RING finger protein RNF-5 in C. elegans. |
ジャーナル | J Cell Biol |
Abstract |
Here, we describe a new muscle LIM domain protein, UNC-95, and identify it as a novel target for the RING finger protein RNF-5 in the Caenorhabditis elegans body wall muscle. unc-95(su33) animals have disorganized muscle actin and myosin-containing filaments as a result of a failure to assemble normal muscle adhesion structures. UNC-95 is active downstream of PAT-3/beta-integrin in the assembly pathways of the muscle dense body and M-line attachments, and upstream of DEB-1/vinculin in the dense body assembly pathway. The translational UNC-95::GFP fusion construct is expressed in dense bodies, M-lines, and muscle-muscle cell boundaries as well as in muscle cell bodies. UNC-95 is partially colocalized with RNF-5 in muscle dense bodies and its expression and localization are regulated by RNF-5. rnf-5(RNAi) or a RING domain deleted mutant, rnf-5(tm794), exhibit structural defects of the muscle attachment sites. Together, our data demonstrate that UNC-95 constitutes an essential component of muscle adhesion sites that is regulated by RNF-5. |
巻・号 | 165(6) |
ページ | 857-67 |
公開日 | 2004-6-21 |
DOI | 10.1083/jcb.200401133 |
PII | jcb.200401133 |
PMID | 15210732 |
PMC | PMC2172400 |
MeSH | Amino Acid Sequence Animals Base Sequence Binding Sites Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / chemistry Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Adhesion Intracellular Signaling Peptides and Proteins Molecular Sequence Data Muscles / physiology Protein Biosynthesis RNA, Antisense / genetics RNA, Antisense / metabolism RNA, Small Interfering Recombinant Fusion Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic |
IF | 8.811 |
引用数 | 34 |
WOS 分野 | CELL BIOLOGY |
リソース情報 | |
線虫 | tm794 |