RRC ID 42101
Author Miyoshi C, Taguchi E, Ohshima N.
Title Leukocyte behavior in angiogenic vessels is affected by tumor-derived nitric oxide.
Journal Jpn. J. Physiol.
Abstract Recent studies indicate a possible role of nitric oxide (NO) in regulating leukocyte-endothelial cell interactions, which plays a key role in the tumor immunity. The purpose of the present study is aimed to observe the tumor hemodynamics intravitally and to clarify the effect of NO on tumor microcirculation by means of a real-time confocal laser-scanning microscope using NO-reactive indicators. Visualization of localization of NO and the leukocyte behavior was made in the mesenteric microvessels of an experimental tumor model rat. Production of NO was clearly visualized along the endothelium of the tumor-free rats, but scarcely found in the newly formed tumor microvessels. A higher level of NO production was observed in a solid tumor region, where a more marked decrease in the leukocyte-endothelial cell interactions was observed. Local administration of a NO synthase (NOS) inhibitor increased leukocyte adhesion. This indicates that tumor-derived NOS creates the tumor specific microenvironment of the immature angiogenic tumor vessels, thereby modulating leukocyte behavior.
Volume 53(5)
Pages 343-50
Published 2003-10
DOI 10.2170/jjphysiol.53.343
PMID 14975180
MeSH Animals Cell Adhesion Disease Models, Animal Endothelium, Vascular / physiology Enzyme Inhibitors / pharmacology Fluorescein Fluorescent Dyes Hemodynamics / physiology Leukocytes / physiology* Male Mesentery / blood supply* Microcirculation / physiopathology Microscopy, Confocal NG-Nitroarginine Methyl Ester / pharmacology Neovascularization, Pathologic / pathology Neovascularization, Pathologic / physiopathology* Nitric Oxide / metabolism Nitric Oxide / physiology* Peritoneal Neoplasms / blood supply* Peritoneal Neoplasms / physiopathology Rats Rats, Inbred F344
Times Cited 1
Human and Animal Cells