RRC ID 42107
著者 Adachi M, Koyama H, Long Z, Sekine M, Furuchi T, Imai K, Nimura N, Shimamoto K, Nakajima T, Homma H.
タイトル L-Glutamate in the extracellular space regulates endogenous D-aspartate homeostasis in rat pheochromocytoma MPT1 cells.
ジャーナル Arch Biochem Biophys
Abstract In previous studies [FEBS Lett. 434 (1998) 231, Arch. Biochem. Biophys. 404 (2002) 92], we demonstrated for the first time that D-aspartate (D-Asp) is synthesized in cultured mammalian cell lines, such as pheochromocytoma 12 (PC12) and its subclone, MPT1. Our current focus is analysis of the dynamics of D-Asp homeostasis in these cells. In this communication, we show that L-glutamate (Glu) and L-Glu transporter substrates in the extracellular space regulate the homeostasis of endogenous D-Asp in MPT1 cells. D-Asp is apparently in dynamic homeostasis, whereby endogenous D-Asp is constantly released into the extracellular space by an undefined mechanism, and continuously and intensively taken up into cells by an L-Glu transporter. Under these conditions, L-Glu and its transporter substrates in the medium may competitively inhibit the uptake of D-Asp via the transporter, resulting in accumulation of the amino acid in the extracellular space. We additionally demonstrate that DL-TBOA, a well-established L-Glu transporter inhibitor, is taken up by the transporter during long time intervals, but not on a short time-scale.
巻・号 424(1)
ページ 89-96
公開日 2004-4-1
DOI 10.1016/j.abb.2004.01.016
PII S0003986104000529
PMID 15019840
MeSH Amino Acid Transport System X-AG / antagonists & inhibitors Amino Acid Transport System X-AG / metabolism Animals Aspartic Acid / analogs & derivatives Aspartic Acid / metabolism* Aspartic Acid / pharmacology Cell Line, Tumor Cysteine / analogs & derivatives* Cysteine / metabolism Cysteine / pharmacology Extracellular Space / metabolism Glutamic Acid / metabolism* Glutamic Acid / pharmacology Homeostasis Neurotransmitter Agents PC12 Cells Pheochromocytoma / metabolism Rats
IF 3.391
引用数 16
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)