RRC ID |
42150
|
Author |
Nakamori M, Fu X, Rousseau R, Chen SY, Zhang X.
|
Title |
Destruction of nonimmunogenic mammary tumor cells by a fusogenic oncolytic herpes simplex virus induces potent antitumor immunity.
|
Journal |
Mol Ther
|
Abstract |
In principle, destruction of tumor cells in vivo by oncolytic agents would release the entire repertoire of tumor antigens in their natural forms, leading to effective antitumor immunity. This goal has been elusive despite extensive testing of numerous strategies. We developed a doubly fusogenic oncolytic herpes simplex virus (Synco-2D) that kills tumor cells by a unique dual mechanism combining direct cytolysis with syncytial formation induced by cell membrane fusion. A single intratumor injection of Synco-2D induced strong antitumor immunity against an otherwise nonimmunogenic murine mammary tumor growing in immune-competent mice. CD8+ T cells were the primary mediators of immunity, contributing to the destruction of both primary and metastatic tumors. We conclude that the fusogenic capacity of Synco-2D enables it to elicit antitumor immunity exceeding that induced by more conventional oncolytic viruses.
|
Volume |
9(5)
|
Pages |
658-65
|
Published |
2004-5-1
|
DOI |
10.1016/j.ymthe.2004.02.019
|
PII |
S1525-0016(04)00085-1
|
PMID |
15120326
|
MeSH |
Animals
Carcinoma / immunology
Carcinoma / virology
Cell Line
Cell Survival
Female
Interferon-gamma / immunology
Interleukin-10 / immunology
Lung Neoplasms / pathology
Lung Neoplasms / secondary
Lymphocyte Depletion
Mammary Neoplasms, Experimental / immunology*
Mammary Neoplasms, Experimental / virology
Membrane Fusion
Mice
Mice, Inbred BALB C
Neoplasm Metastasis / pathology
Neoplasm Transplantation
Simplexvirus / physiology*
T-Lymphocytes, Cytotoxic / immunology
Tumor Escape / immunology
|
IF |
8.986
|
Times Cited |
47
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
Resource |
Human and Animal Cells |
Meth-A(RCB0464) |