| RRC ID |
42154
|
| 著者 |
Tomatsu M, Mujin T, Shibamoto N, Tashiro F, Ikuta A.
|
| タイトル |
Production of aralin, a selective cytotoxic lectin against human transformed cells, in callus culture of Aralia elata.
|
| ジャーナル |
Planta Med
|
| Abstract |
Recently we found that aralin, a novel lectin, from Aralia elata selectively induces apoptosis in human virus-transformed and cancer cells compared with normal cells. To overcome the reduction of aralin biosynthesis according to the development of leaves, we established callus tissues from the young shoots. The N-terminal amino acid sequence and immunochemical analyses revealed that ca-aralin, an aralin synthesized in the callus, was structurally identical with aralin except for the glycosylation of the B chain. The selective cytotoxic index (ratio of IC50 value against human lung normal cells versus virus-transformed cells) of ca-aralin was 2-fold higher than that of aralin. Thus, these results suggest that ca-aralin is more useful than aralin as an anticancer agent.
|
| 巻・号 |
70(5)
|
| ページ |
469-71
|
| 公開日 |
2004-5-1
|
| DOI |
10.1055/s-2004-818979
|
| PMID |
15124096
|
| MeSH |
Antineoplastic Agents, Phytogenic / administration & dosage
Antineoplastic Agents, Phytogenic / pharmacology*
Antineoplastic Agents, Phytogenic / therapeutic use
Aralia*
Cell Transformation, Viral / drug effects
Culture Techniques
Humans
Inhibitory Concentration 50
Lectins / administration & dosage
Lectins / pharmacology*
Lectins / therapeutic use
Lung / cytology
Lung / drug effects
Phytotherapy*
Plant Extracts / administration & dosage
Plant Extracts / pharmacology*
Plant Extracts / therapeutic use
Plant Shoots
Ribosome Inactivating Proteins, Type 2
Tumor Cells, Cultured / drug effects
|
| IF |
2.687
|
| 引用数 |
5
|
|
WOS 分野
|
PLANT SCIENCES
PHARMACOLOGY & PHARMACY
INTEGRATIVE & COMPLEMENTARY MEDICINE
CHEMISTRY, MEDICINAL
|
| リソース情報 |
| ヒト・動物細胞 |
WI-38
VA-13(RCB0251) |
