論文 - 詳細
| RRC ID | 42169 |
|---|---|
| 著者 | Shimomura K, Sakakura C, Takemura M, Takagi T, Fukuda K, Kin S, Nakase Y, Miyagawa K, Ohgaki M, Fujiyama J, Fujita Y, Nakanishi M, Hagiwara A, Shirane M, Okazaki Y, Hayashizaki Y, Yamagishi H. |
| タイトル | Combination of L-3-phosphoserine phosphatase and CEA using real-time RT-PCR improves accuracy in detection of peritoneal micrometastasis of gastric cancer. |
| ジャーナル | Anticancer Res |
| Abstract |
Peritoneal metastasis is the most frequent form of recurrence for advanced gastric cancer. We previously performed a global analysis of the gene expression of gastric cancer cell lines established from peritoneal metastasis with cDNA microarray. One of the up-regulated genes is L-3-phosphoserine phosphatase (L3-PP). We have examined its potential as a novel marker for the detection of peritoneal micrometastasis of gastric cancer. L3-PP mRNA in peritoneal wash from 88 gastric cancer patients was quantified for comparison of carcinoembryonic antigen (CEA) mRNA by means of real-time RT-PCR with a fluorescently-labeled probe to predict peritoneal recurrence. The quantity of L3-PP and CEA correlated with wall penetration. The cut-off value was set at the upper limit of the quantitative value of T1 cases (tumor invades within submucosa) and those above the cut-off value constituted the micrometastasis (MM+) group; eight out of 14 cases with peritoneal dissemination were MM+ L3-PP (57.1% sensitivity) and two out of 57 T1 and T2 cases were MM+ (93% specificity). For two out of 14 cases of peritoneal dissemination only L3-PP could detect micrometastasis of gastric cancer, indicating that L3-PP is superior to CEA especially in poorly-differentiated adenocarcinoma. The combination of CEA and L3-PP improved the accuracy of diagnosis up to 85.7%. Consequently, free cancer cells that cannot be detected by CEA mRNA could be detected using L3-PP mRNA. CEA alone was not sufficient, but L3-PP and CEA in combination can attain a higher accuracy of detection. |
| 巻・号 | 24(2C) |
| ページ | 1113-20 |
| 公開日 | 2004-1-1 |
| PMID | 15154633 |
| MeSH | Actins / biosynthesis Actins / genetics Aged Aged, 80 and over Biomarkers, Tumor / biosynthesis* Biomarkers, Tumor / genetics Carcinoembryonic Antigen / biosynthesis* Carcinoembryonic Antigen / genetics Cell Line, Tumor False Negative Reactions Female Humans Male Middle Aged Peritoneal Neoplasms / diagnosis Peritoneal Neoplasms / enzymology Peritoneal Neoplasms / immunology Peritoneal Neoplasms / secondary* Phosphoric Monoester Hydrolases / biosynthesis* Phosphoric Monoester Hydrolases / genetics RNA, Messenger / biosynthesis RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction / methods* Sensitivity and Specificity Stomach Neoplasms / enzymology Stomach Neoplasms / immunology Stomach Neoplasms / pathology* |
| IF | 1.994 |
| 引用数 | 12 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |