論文 - 詳細
| RRC ID | 42186 |
|---|---|
| 著者 | Eelen G, Verlinden L, Van Camp M, Mathieu C, Carmeliet G, Bouillon R, Verstuyf A. |
| タイトル | Microarray analysis of 1alpha,25-dihydroxyvitamin D3-treated MC3T3-E1 cells. |
| ジャーナル | J Steroid Biochem Mol Biol |
| Abstract |
The active form of Vitamin D, 1alpha,25-dihydroxyvitamin D(3) [1,25-(OH)(2)D(3)], demonstrates potent antiproliferative actions on normal as well as on malignant cell types by blocking the transition from the G1- to the S-phase of the cell cycle. Key target genes for 1,25-(OH)(2)D(3) in this non-classic effect remain largely unknown. Therefore, this study aims to identify genes that, through changes in expression after 1,25-(OH)(2)D(3) treatment, contribute to the observed antiproliferative effect. cDNA microarrays containing 4600 genes were used to investigate changes in gene expression in MC3T3-E1 mouse osteoblasts at 6 and at 12h after treatment with 1,25-(OH)(2)D(3) (10(-8)M), preceding (6h) or coinciding with (12h) the G1/S block in these cells. Approximately one fifth of the genes that were significantly down-regulated after a 12h incubation period with 1,25-(OH)(2)D(3) were genes involved in the DNA replication process, a basic process for cell growth that starts at the end of G1-phase and continues in S-phase. Down-regulation of these genes by 1,25-(OH)(2)D(3) was confirmed by quantitative RT-PCR in MC3T3-E1. In conclusion, cDNA microarrays revealed that treatment of MC3T3-E1 cells with 1,25-(OH)(2)D(3) resulted in the down-regulation of DNA replication genes in parallel with the observed G1/S-arrest. |
| 巻・号 | 89-90(1-5) |
| ページ | 405-7 |
| 公開日 | 2004-5-1 |
| DOI | 10.1016/j.jsbmb.2004.03.008 |
| PII | S0960076004000536 |
| PMID | 15225810 |
| MeSH | 3T3 Cells Animals Calcitriol / pharmacology* Cell Division / drug effects* Gene Expression Regulation / drug effects Mice Oligonucleotide Array Sequence Analysis* Reverse Transcriptase Polymerase Chain Reaction |
| IF | 3.813 |
| 引用数 | 25 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |