RRC ID |
4218
|
Author |
Cui M, Allen MA, Larsen A, Macmorris M, Han M, Blumenthal T.
|
Title |
Genes involved in pre-mRNA 3'-end formation and transcription termination revealed by a lin-15 operon Muv suppressor screen.
|
Journal |
Proc Natl Acad Sci U S A
|
Abstract |
RNA polymerase II (Pol II) transcription termination involves two linked processes: mRNA 3'-end formation and release of Pol II from DNA. Signals for 3' processing are recognized by a protein complex that includes cleavage polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF). Here we identify suppressors encoding proteins that play roles in processes at the 3' ends of genes by exploiting a mutation in which the 3' end of another gene is transposed into the first gene of the Caenorhabditis elegans lin-15 operon. As expected, genes encoding CPSF and CstF were identified in the screen. We also report three suppressors encoding proteins containing a domain that interacts with the C-terminal domain of Pol II (CID). We show that two of the CID proteins are needed for efficient 3' cleavage and thus may connect transcription termination with RNA cleavage. Furthermore, our results implicate a serine/arginine-rich (SR) protein, SRp20, in events following 3'-end cleavage, leading to termination of transcription.
|
Volume |
105(43)
|
Pages |
16665-70
|
Published |
2008-10-28
|
DOI |
10.1073/pnas.0807104105
|
PII |
0807104105
|
PMID |
18946043
|
PMC |
PMC2571909
|
MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins*
Cleavage And Polyadenylation Specificity Factor / genetics
Cleavage And Polyadenylation Specificity Factor / metabolism
Cleavage Stimulation Factor / genetics
Cleavage Stimulation Factor / metabolism
Operon
RNA 3' End Processing*
RNA Polymerase II / metabolism
RNA Precursors / genetics*
RNA-Binding Proteins
Terminator Regions, Genetic
Transcription Factors*
Transcription, Genetic*
|
IF |
9.412
|
Times Cited |
38
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
C.elegans |
tm2715
tm2802
tm367 |