| RRC ID |
42198
|
| 著者 |
Kawazoe Y, Shiba T, Nakamura R, Mizuno A, Tsutsumi K, Uematsu T, Yamaoka M, Shindoh M, Kohgo T.
|
| タイトル |
Induction of calcification in MC3T3-E1 cells by inorganic polyphosphate.
|
| ジャーナル |
J Dent Res
|
| Abstract |
Relatively large amounts of inorganic polyphosphate [poly(P)] (400 microM) have been found in normal osteoblasts. The effect of poly(P) with an average chain length of 65 phosphate residues on cell calcification was therefore investigated with the use of MC3T3-E1 cells. Expression of both osteopontin and osteocalcin was induced by poly(P) (0.1 approximately 1 mM), and cells treated with poly(P) were strongly stained by alizarin red. In addition, the level of alkaline phosphatase activity induced in poly(P)-treated cells was two-fold higher than that in either orthophosphate-treated or control cells but not higher than that in cells treated with beta-glycerophosphate and ascorbic acid. In contrast, however, polyphosphatase activities were activated by poly(P) treatment to levels up to six-fold greater than that in controls. MC3T3-E1 cells may utilize poly(P) as a phosphate source for calcification rather than phosphate sources that are mainly produced by ALPase. Poly(P)-dependent induction of polyphosphatase activities may therefore promote calcification in MC3T3-E1 cells.
|
| 巻・号 |
83(8)
|
| ページ |
613-8
|
| 公開日 |
2004-8-1
|
| DOI |
10.1177/154405910408300806
|
| PII |
83/8/613
|
| PMID |
15271969
|
| MeSH |
3T3 Cells
Alkaline Phosphatase
Animals
Biomarkers
Calcification, Physiologic / drug effects*
Calcification, Physiologic / physiology
Cell Differentiation / drug effects
Inorganic Chemicals / pharmacology
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Osteoblasts / cytology
Osteoblasts / drug effects*
Osteoblasts / metabolism
Osteocalcin / drug effects*
Osteocalcin / metabolism
Osteopontin
Polyphosphates / pharmacology*
Sialoglycoproteins / drug effects*
Sialoglycoproteins / metabolism
Skull / cytology
Skull / drug effects*
Skull / metabolism
|
| IF |
4.914
|
| 引用数 |
70
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126)
BALB/3T3 clone A31(RCB0005) |
