RRC ID 42198
Author Kawazoe Y, Shiba T, Nakamura R, Mizuno A, Tsutsumi K, Uematsu T, Yamaoka M, Shindoh M, Kohgo T.
Title Induction of calcification in MC3T3-E1 cells by inorganic polyphosphate.
Journal J Dent Res
Abstract Relatively large amounts of inorganic polyphosphate [poly(P)] (400 microM) have been found in normal osteoblasts. The effect of poly(P) with an average chain length of 65 phosphate residues on cell calcification was therefore investigated with the use of MC3T3-E1 cells. Expression of both osteopontin and osteocalcin was induced by poly(P) (0.1 approximately 1 mM), and cells treated with poly(P) were strongly stained by alizarin red. In addition, the level of alkaline phosphatase activity induced in poly(P)-treated cells was two-fold higher than that in either orthophosphate-treated or control cells but not higher than that in cells treated with beta-glycerophosphate and ascorbic acid. In contrast, however, polyphosphatase activities were activated by poly(P) treatment to levels up to six-fold greater than that in controls. MC3T3-E1 cells may utilize poly(P) as a phosphate source for calcification rather than phosphate sources that are mainly produced by ALPase. Poly(P)-dependent induction of polyphosphatase activities may therefore promote calcification in MC3T3-E1 cells.
Volume 83(8)
Pages 613-8
Published 2004-8-1
DOI 10.1177/154405910408300806
PII 83/8/613
PMID 15271969
MeSH 3T3 Cells Alkaline Phosphatase Animals Biomarkers Calcification, Physiologic / drug effects* Calcification, Physiologic / physiology Cell Differentiation / drug effects Inorganic Chemicals / pharmacology Mice Mice, Inbred BALB C Mice, Inbred Strains Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteocalcin / drug effects* Osteocalcin / metabolism Osteopontin Polyphosphates / pharmacology* Sialoglycoproteins / drug effects* Sialoglycoproteins / metabolism Skull / cytology Skull / drug effects* Skull / metabolism
IF 4.914
Times Cited 70
Human and Animal Cells MC3T3-E1(RCB1126) BALB/3T3 clone A31(RCB0005)