Reference - Detail
| RRC ID | 42201 |
|---|---|
| Author | Enomoto M, Endo D, Kawashima S, Park MK. |
| Title | Human type II GnRH receptor mediates effects of GnRH on cell proliferation. |
| Journal | Zoolog Sci |
| Abstract |
GnRH (gonadotropin-releasing hormone) is well-known as the central regulator of the reproductive system through its stimulation of gonadotropin release from the pituitary. Progress in studies on GnRH demonstrated that GnRH has both inhibitory and stimulatory effects on cell proliferation depending on the cell type, and the mechanisms of these effects have been intensively studied. However, even human GnRH receptors which mediate GnRH stimulation have not been completely identified. In the present study, we showed that the inhibitory and stimulatory effects of GnRH on colony-formation using four cell lines and have demonstrated that the inhibitory and stimulatory effects of GnRH exhibit distinctly different patterns of ligand sensitivity. This result strongly suggests that the two opposite effects of GnRH occur via different types of GnRH receptors, however expressional analyses of human GnRH receptors did not exhibit the significantly different pattern between negatively and positively responding cell lines. Then, in order to identify the GnRH receptors involved in the two opposite effects, effects of GnRH were analysed under the conditions that human GnRH receptors were knocked down by the technique of RNA interference. Consequently, it was found that human type II GnRH receptor mediates GnRH stimulation and its splice variant determines the direction of the response to GnRH. These results are the first clear evidence for the functionality of human type II GnRH receptor. Therefore our novel findings are quite noticeable and will greatly contribute to the studies on the mechanisms of the effects of GnRH on cell proliferation in the future. |
| Volume | 21(7) |
| Pages | 763-70 |
| Published | 2004-7-1 |
| DOI | 10.2108/zsj.21.763 |
| PMID | 15277720 |
| MeSH | Base Sequence Cell Division / drug effects Cell Division / physiology DNA Primers Gonadotropin-Releasing Hormone / metabolism* Gonadotropin-Releasing Hormone / pharmacology* Humans Ligands Molecular Sequence Data RNA Interference RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Receptors, LHRH / metabolism* Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured |
| IF | 0.843 |
| Times Cited | 22 |
| WOS Category | ZOOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Jurkat(RCB0806) HHUA(RCB0658) |