| RRC ID |
42212
|
| 著者 |
Arakawa Y, Kajino K, Kano S, Tobita H, Hayashi J, Yasen M, Moriyama M, Arakawa Y, Hino O.
|
| タイトル |
Transcription of dbpA, a Y box binding protein, is positively regulated by E2F1: implications in hepatocarcinogenesis.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Human hepatocellular carcinoma is one of the most common cancers in the world. We previously showed that dbpA, a member of the Y box family of proteins, could accelerate the process of inflammation-induced hepatocarcinogenesis, and that dbpA is more abundantly expressed in hepatocellular carcinoma than in non-tumorous tissue. In this study, to clarify the mechanism by which expression of dbpA is enhanced in the proliferative state, we examined the transcriptional activity of the dbpA promoter region. We focused on the sequence 5'-TTTGGGGC-3' (-8 to -1 in the promoter region) resembling the E2F binding site (one base mismatch, TFSEARCH score 86.2). By overexpressing E2F1 in Huh-7 cells, transcriptional activity of dbpA was significantly increased, and this increase was abolished by mutating or deleting this sequence. Thus, expression of dbpA was positively regulated by E2F1, suggesting that one of the effects of E2F1 on cell proliferation might be mediated by dbpA at the carcinogenesis step.
|
| 巻・号 |
322(1)
|
| ページ |
297-302
|
| 公開日 |
2004-9-10
|
| DOI |
10.1016/j.bbrc.2004.04.208
|
| PII |
S0006-291X(04)01568-2
|
| PMID |
15313206
|
| MeSH |
Animals
CCAAT-Enhancer-Binding Proteins
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Line, Tumor
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
E2F Transcription Factors
E2F1 Transcription Factor
Gene Expression Regulation, Neoplastic / genetics
Heat-Shock Proteins / genetics
Heat-Shock Proteins / metabolism*
Hepatectomy
Humans
Liver / metabolism*
Liver / surgery
Liver Neoplasms / genetics
Liver Neoplasms / metabolism*
Male
Mice
Mice, Inbred C57BL
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcriptional Activation*
|
| IF |
2.985
|
| 引用数 |
33
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7 |
