RRC ID 42256
Author Wakebayashi D, Nishiyama N, Itaka K, Miyata K, Yamasaki Y, Harada A, Koyama H, Nagasaki Y, Kataoka K.
Title Polyion complex micelles of pDNA with acetal-poly(ethylene glycol)-poly(2-(dimethylamino)ethyl methacrylate) block copolymer as the gene carrier system: physicochemical properties of micelles relevant to gene transfection efficacy.
Journal Biomacromolecules
Abstract or = 3 had a constant size of approximately 90-100 nm. Eventually, acetal-PEG-PAMA/pDNA micelles underwent no precipitation even after long-term storage for more than 1 month at all N/P ratios. The PIC micelles were stable even in the presence of excess polyanions, poly(vinyl sulfate), in contrast to polyplexes based on the PAMA homopolymer, yet this stabilization effect was highly dependent on the N/P ratio to reach a plateau at N/P = 3-4. This character may be attributed to the increased hydrophobicity in the vicinity of the complexed pDNA. Furthermore, the pDNA in the micelle was adequately protected from DNase I attack. The transfection ability of the PIC micelles toward 293 cells was remarkably enhanced with an increasing N/P ratio as high as 25. The zeta-potential of the micelles with a high N/P ratio was an appreciably large positive value, suggesting a noncooperative micelle formation. This deviated micellar composition with an excess cationic nature as well as the presence of free acetal-PEG-PAMA may play a substantial role in the enhanced transfection efficiency of the PIC micelle system in the high N/P ratio (approximately 25) region.
Volume 5(6)
Pages 2128-36
Published 2004-11
DOI 10.1021/bm040009j
PMID 15530026
MeSH Cations Cell Line DNA / chemistry Deoxyribonuclease I / metabolism Escherichia coli / metabolism Ethidium / pharmacology Genetic Vectors Humans Light Macromolecular Substances Methacrylates / chemistry Micelles* Plasmids / chemistry* Plasmids / metabolism Polyethylene Glycols / chemistry Polymers / chemistry* Scattering, Radiation Static Electricity Time Factors Transfection
IF 5.738
Times Cited 57
WOS Category CHEMISTRY, ORGANIC POLYMER SCIENCE BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells