RRC ID 42257
Author Iwata R, Suk-In P, Hoven VP, Takahara A, Akiyoshi K, Iwasaki Y.
Title Control of nanobiointerfaces generated from well-defined biomimetic polymer brushes for protein and cell manipulations.
Journal Biomacromolecules
Abstract To better understand protein/material and cell/material interactions at the submolecular level, well-defined polymer brushes consisting of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) on silicon wafers were prepared by atom transfer radical polymerization (ATRP). Silicon wafers were treated with 3-(2-bromoisobutyryl)propyl dimethylchlorosilane (BDCS) to form a monolayer that acts as initiators for ATRP. Silicon-supported BDCS monolayers were soaked in a methanol/water mixture solution containing Cu(I)Br, bipyridine, and a sacrificial initiator. After MPC was added to the solution, ATRP was carried out for 18 h. The molecular weight and thickness of the PMPC brush layer on the silicon surface increased with an increase in the polymerization time. The dense polymer brushes were obtained by the "grafting from" system. By selective decomposition of the BDCS monolayer by UV light-irradiation, the PMPC brush region and the sizes were well controlled, resulting in fabricating micropatterns of the PMPC brushes. When the thickness of the PMPC brush layer was greater than 5.5 +/- 1.0 nm (3 h polymerization), serum protein adsorption and fibroblast adhesion were effectively reduced, i.e., proteins and cells could recognize such thin polymer brushes on the surface. In addition, the density of the adherent cells on the patterned PMPC brush surface could be controlled by changing the size of the pattern.
Volume 5(6)
Pages 2308-14
Published 2004-11-1
DOI 10.1021/bm049613k
PMID 15530046
MeSH Adsorption Animals Biocompatible Materials / chemistry* Cell Adhesion Cell Line Electron Probe Microanalysis Macromolecular Substances Mice Microscopy, Atomic Force Microscopy, Fluorescence Models, Chemical Nanotechnology / methods* Polymers / chemistry* Proteins / chemistry* Silicon / chemistry Surface Properties Time Factors Ultraviolet Rays Water
IF 5.667
Times Cited 240
Human and Animal Cells