論文 - 詳細
| RRC ID | 42257 |
|---|---|
| 著者 | Iwata R, Suk-In P, Hoven VP, Takahara A, Akiyoshi K, Iwasaki Y. |
| タイトル | Control of nanobiointerfaces generated from well-defined biomimetic polymer brushes for protein and cell manipulations. |
| ジャーナル | Biomacromolecules |
| Abstract |
To better understand protein/material and cell/material interactions at the submolecular level, well-defined polymer brushes consisting of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) on silicon wafers were prepared by atom transfer radical polymerization (ATRP). Silicon wafers were treated with 3-(2-bromoisobutyryl)propyl dimethylchlorosilane (BDCS) to form a monolayer that acts as initiators for ATRP. Silicon-supported BDCS monolayers were soaked in a methanol/water mixture solution containing Cu(I)Br, bipyridine, and a sacrificial initiator. After MPC was added to the solution, ATRP was carried out for 18 h. The molecular weight and thickness of the PMPC brush layer on the silicon surface increased with an increase in the polymerization time. The dense polymer brushes were obtained by the "grafting from" system. By selective decomposition of the BDCS monolayer by UV light-irradiation, the PMPC brush region and the sizes were well controlled, resulting in fabricating micropatterns of the PMPC brushes. When the thickness of the PMPC brush layer was greater than 5.5 +/- 1.0 nm (3 h polymerization), serum protein adsorption and fibroblast adhesion were effectively reduced, i.e., proteins and cells could recognize such thin polymer brushes on the surface. In addition, the density of the adherent cells on the patterned PMPC brush surface could be controlled by changing the size of the pattern. |
| 巻・号 | 5(6) |
| ページ | 2308-14 |
| 公開日 | 2004-1-1 |
| DOI | 10.1021/bm049613k |
| PMID | 15530046 |
| MeSH | Adsorption Animals Biocompatible Materials / chemistry* Cell Adhesion Cell Line Electron Probe Microanalysis Macromolecular Substances Mice Microscopy, Atomic Force Microscopy, Fluorescence Models, Chemical Nanotechnology / methods* Polymers / chemistry* Proteins / chemistry* Silicon / chemistry Surface Properties Time Factors Ultraviolet Rays Water |
| IF | 6.092 |
| 引用数 | 240 |
| WOS 分野 | CHEMISTRY, ORGANIC POLYMER SCIENCE BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929 |