論文 - 詳細
| RRC ID | 42271 |
|---|---|
| 著者 | Hasuda H, Kwon OH, Kang IK, Ito Y. |
| タイトル | Synthesis of photoreactive pullulan for surface modification. |
| ジャーナル | Biomaterials |
| Abstract |
Photoreactive pullulan was prepared, the polymer was photoimmobilized on polymeric or organic surfaces, and its interactions with a protein and a cell type were investigated. The photoreactive pullulan was synthesized by a coupling reaction with 4-azidobenzonic acid. Surface modification was carried out in the presence or absence of a micropatterned photomask containing 100 microm transparent stripes with 150 microm gaps, making it easy to confirm the immobilization. By the micropatterning method, immobilization of the photoreactive pullulan on polystyrene, polyethylene, and silane-coupled glass was confirmed. Contact angles were measured on the unpatterned surfaces. Although the original surfaces have different contact angles, the contact angle on Az-pullulan-immobilized surface was the same on all surfaces. This result demonstrated that photoimmobilization completely covered the surface with Az-pullulan. Protein adsorption was investigated using fluorescently labeled albumin applied to the micropatterned surface: fluorescence microscopy demonstrated that adsorption was reduced on the pullulan-immobilized regions. Culture of RAW264 cells, derived from mouse leukemic monocytes, on the micropatterned surface for 22 h showed that cells did not adhere to the immobilized pullulan regions. In conclusion, photoreactive pullulan was covalently immobilized on various surfaces and tended to reduce interactions with proteins and cells. |
| 巻・号 | 26(15) |
| ページ | 2401-6 |
| 公開日 | 2005-5-1 |
| DOI | 10.1016/j.biomaterials.2004.07.065 |
| PII | S0142-9612(04)00702-1 |
| PMID | 15585243 |
| MeSH | Adsorption Animals Azides / chemistry Blood Proteins / chemistry* Cell Adhesion / drug effects Cell Line, Tumor Coated Materials, Biocompatible / chemistry Coated Materials, Biocompatible / pharmacology* Coated Materials, Biocompatible / radiation effects Glucans / chemistry* Glucans / pharmacology* Glucans / radiation effects Macrophages / cytology* Macrophages / drug effects* Macrophages / physiology Materials Testing Mice Photochemistry / methods* Protein Binding Surface Properties Ultraviolet Rays |
| IF | 10.317 |
| 引用数 | 43 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |