RRC ID 42278
Author Haga H, Irahara C, Kobayashi R, Nakagaki T, Kawabata K.
Title Collective movement of epithelial cells on a collagen gel substrate.
Journal Biophys. J.
Abstract Collective cell movement acts as an efficient strategy in many physiological events, including wound healing, embryonic development, and morphogenesis. We found that epithelial cells (Madin-Darby canine kidney cell) migrated collectively along one direction on a collagen gel substrate. Time-lapse images of Madin-Darby canine kidney cells cultured on type-I collagen gels and glass substrates were captured by phase contrast microscopy equipped with an incubation system. On the gel substrate, the directions of cell movement gradually converged on one direction as the number of cells increased, whereas the cells moved randomly on the glass substrate. We also observed "leader" cells, which extended large lamellae and were accompanied by many "follower" cells, migrating in the direction of oriented collagen fibers. The mean-squared displacement of each cell movement and the spatial correlation function calculated from the spatial distribution of cell velocity were obtained as functions of observation time. In the case of the gel substrate, the spatial correlation length increased gradually, representing the collectiveness of multicellular movement.
Volume 88(3)
Pages 2250-6
Published 2005-3
DOI 10.1529/biophysj.104.047654
PII S0006-3495(05)73284-7
PMID 15596493
PMC PMC1305274
MeSH Animals Cell Aggregation / drug effects Cell Aggregation / physiology* Cell Communication / drug effects Cell Communication / physiology* Cell Culture Techniques / methods Cell Line Cell Movement / drug effects Cell Movement / physiology* Coated Materials, Biocompatible / pharmacology Collagen Type I / pharmacology* Dogs Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / physiology* Gels Kidney / cytology* Kidney / drug effects Kidney / physiology* Microscopy, Phase-Contrast Microscopy, Video
IF 3.495
Times Cited 67
WOS Category BIOPHYSICS
Resource
Human and Animal Cells