論文 - 詳細
| RRC ID | 42278 |
|---|---|
| 著者 | Haga H, Irahara C, Kobayashi R, Nakagaki T, Kawabata K. |
| タイトル | Collective movement of epithelial cells on a collagen gel substrate. |
| ジャーナル | Biophys J |
| Abstract |
Collective cell movement acts as an efficient strategy in many physiological events, including wound healing, embryonic development, and morphogenesis. We found that epithelial cells (Madin-Darby canine kidney cell) migrated collectively along one direction on a collagen gel substrate. Time-lapse images of Madin-Darby canine kidney cells cultured on type-I collagen gels and glass substrates were captured by phase contrast microscopy equipped with an incubation system. On the gel substrate, the directions of cell movement gradually converged on one direction as the number of cells increased, whereas the cells moved randomly on the glass substrate. We also observed "leader" cells, which extended large lamellae and were accompanied by many "follower" cells, migrating in the direction of oriented collagen fibers. The mean-squared displacement of each cell movement and the spatial correlation function calculated from the spatial distribution of cell velocity were obtained as functions of observation time. In the case of the gel substrate, the spatial correlation length increased gradually, representing the collectiveness of multicellular movement. |
| 巻・号 | 88(3) |
| ページ | 2250-6 |
| 公開日 | 2005-3-1 |
| DOI | 10.1529/biophysj.104.047654 |
| PII | S0006-3495(05)73284-7 |
| PMID | 15596493 |
| PMC | PMC1305274 |
| MeSH | Animals Cell Aggregation / drug effects Cell Aggregation / physiology* Cell Communication / drug effects Cell Communication / physiology* Cell Culture Techniques / methods Cell Line Cell Movement / drug effects Cell Movement / physiology* Coated Materials, Biocompatible / pharmacology Collagen Type I / pharmacology* Dogs Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / physiology* Gels Kidney / cytology* Kidney / drug effects Kidney / physiology* Microscopy, Phase-Contrast Microscopy, Video |
| IF | 3.854 |
| 引用数 | 86 |
| WOS 分野 | BIOPHYSICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |