| RRC ID |
42281
|
| 著者 |
Tazawa S, Yamato T, Fujikura H, Hiratochi M, Itoh F, Tomae M, Takemura Y, Maruyama H, Sugiyama T, Wakamatsu A, Isogai T, Isaji M.
|
| タイトル |
SLC5A9/SGLT4, a new Na+-dependent glucose transporter, is an essential transporter for mannose, 1,5-anhydro-D-glucitol, and fructose.
|
| ジャーナル |
Life Sci
|
| Abstract |
We isolated a cDNA clone of SLC5A9/SGLT4 from human small intestinal full-length cDNA libraries, and functionally characterized it in vitro. The messenger RNA encoding SGLT4 was mainly expressed in the small intestine and kidney, among the human tissues tested. COS-7 cells transiently expressing SGLT4 exhibited Na(+)-dependent alpha-methyl-D-glucopyranoside (AMG) transport activity with an apparent K(m) of 2.6 mM, suggesting that SGLT4 is a low affinity-type transporter. The rank order of naturally occurring sugar analogs for the inhibition of AMG transport was: D-mannose (Man) > D-glucose (Glc) > D-fructose (Fru) = 1,5-anhydro-D-glucitol (1,5AG) > D-galactose (Gal). Recognition of Man as a substrate was confirmed by direct uptake of Man into the cell. COS-7 cells expressing a putative murine SGLT4 ortholog showed similar Na(+)-dependent AMG transport activity and a similar deduced substrate specificity. These results suggest that SGLT4 would have unique physiological functions (i.e., absorption and/or reabsorption of Man, 1,5AG, and Fru, in addition to Glc).
|
| 巻・号 |
76(9)
|
| ページ |
1039-50
|
| 公開日 |
2005-1-14
|
| DOI |
10.1016/j.lfs.2004.10.016
|
| PII |
S0024-3205(04)00910-5
|
| PMID |
15607332
|
| MeSH |
Amino Acid Sequence
Animals
COS Cells
Deoxyglucose / metabolism*
Fructose / metabolism*
Humans
Mannose / metabolism*
Molecular Sequence Data
Monosaccharide Transport Proteins / genetics
Monosaccharide Transport Proteins / physiology*
RNA, Messenger / analysis
Sodium-Glucose Transporter 2
|
| IF |
3.647
|
| 引用数 |
116
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
MEDICINE, RESEARCH & EXPERIMENTAL
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
