| RRC ID |
42294
|
| 著者 |
Takayama T, Suzuki N, Narukawa M, Goldberg HA, Otsuka K, Ito K.
|
| タイトル |
Enamel matrix derivative is a potent inhibitor of breast cancer cell attachment to bone.
|
| ジャーナル |
Life Sci
|
| Abstract |
This study examined whether enamel matrix derivative (EMD) inhibits the adhesion of cancer cells to bone. A typical breast cancer cell line, MCF-7, was used. Conditioned human osteosarcoma cell (Saos-2) medium was used as extracellular bone matrix (ECBM) to measure cell attachment. MCF-7 cells were incubated on ECBM-coated culture plates with or without soluble EMD, Arg-Gly-Asp (RGD) sequence blocking peptides, recombinant bone sialoprotein (rBSP), or specific integrin antibodies, and the attached cells were quantified using toluidine blue staining. EMD markedly reduced the attachment of MCF-7 cells to ECBM in a dose-dependent manner. An RGD peptide (GRGDSP) and recombinant BSP inhibited cell attachment to the same degree as EMD. Similarly, anti-alphavbeta3 integrin antibody strongly reduced cell attachment, whereas anti-alphavbeta5 and anti-beta1 integrin antibodies had less marked effects on cell attachment. These results show that EMD inhibits MCF-7 cell attachment to a bone matrix and that it might be useful as an anti-adhesive agent for breast cancer cells to bone in vivo.
|
| 巻・号 |
76(11)
|
| ページ |
1211-21
|
| 公開日 |
2005-1-28
|
| DOI |
10.1016/j.lfs.2004.07.025
|
| PII |
S0024-3205(04)00951-8
|
| PMID |
15642592
|
| MeSH |
Antibodies, Monoclonal / pharmacology
Bone Matrix / pathology
Bone Neoplasms / secondary
Bone and Bones / pathology*
Breast Neoplasms / pathology*
Cell Adhesion / drug effects
Cell Culture Techniques
Cell Line, Tumor
Cell Proliferation / drug effects
Culture Media
Dental Enamel Proteins / pharmacology*
Dose-Response Relationship, Drug
Extracellular Matrix / pathology
Humans
Integrin beta1 / immunology
Integrin-Binding Sialoprotein
Integrins / immunology
Oligopeptides / pharmacology
Receptors, Vitronectin / immunology
Recombinant Proteins / pharmacology
Sialoglycoproteins / pharmacology
|
| IF |
3.647
|
| 引用数 |
6
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
|
