RRC ID 42294
Author Takayama T, Suzuki N, Narukawa M, Goldberg HA, Otsuka K, Ito K.
Title Enamel matrix derivative is a potent inhibitor of breast cancer cell attachment to bone.
Journal Life Sci.
Abstract This study examined whether enamel matrix derivative (EMD) inhibits the adhesion of cancer cells to bone. A typical breast cancer cell line, MCF-7, was used. Conditioned human osteosarcoma cell (Saos-2) medium was used as extracellular bone matrix (ECBM) to measure cell attachment. MCF-7 cells were incubated on ECBM-coated culture plates with or without soluble EMD, Arg-Gly-Asp (RGD) sequence blocking peptides, recombinant bone sialoprotein (rBSP), or specific integrin antibodies, and the attached cells were quantified using toluidine blue staining. EMD markedly reduced the attachment of MCF-7 cells to ECBM in a dose-dependent manner. An RGD peptide (GRGDSP) and recombinant BSP inhibited cell attachment to the same degree as EMD. Similarly, anti-alphavbeta3 integrin antibody strongly reduced cell attachment, whereas anti-alphavbeta5 and anti-beta1 integrin antibodies had less marked effects on cell attachment. These results show that EMD inhibits MCF-7 cell attachment to a bone matrix and that it might be useful as an anti-adhesive agent for breast cancer cells to bone in vivo.
Volume 76(11)
Pages 1211-21
Published 2005-1-28
DOI 10.1016/j.lfs.2004.07.025
PII S0024-3205(04)00951-8
PMID 15642592
MeSH Antibodies, Monoclonal / pharmacology Bone Matrix / pathology Bone Neoplasms / secondary Bone and Bones / pathology* Breast Neoplasms / pathology* Cell Adhesion / drug effects Cell Culture Techniques Cell Line, Tumor Cell Proliferation / drug effects Culture Media Dental Enamel Proteins / pharmacology* Dose-Response Relationship, Drug Extracellular Matrix / pathology Humans Integrin beta1 / immunology Integrin-Binding Sialoprotein Integrins / immunology Oligopeptides / pharmacology Receptors, Vitronectin / immunology Recombinant Proteins / pharmacology Sialoglycoproteins / pharmacology
IF 3.448
Times Cited 5
Human and Animal Cells