| RRC ID |
42311
|
| Author |
Zhang K, Washburn NR, Simon CG Jr.
|
| Title |
Cytotoxicity of three-dimensionally ordered macroporous sol-gel bioactive glass (3DOM-BG).
|
| Journal |
Biomaterials
|
| Abstract |
In this study, 80% SiO(2)-20% CaO (mole fraction) three-dimensionally ordered macroporous sol-gel bioactive glass (3DOM-BG, average pore size: 345 nm) particles were prepared and characterized. Since the 3DOM-BGs have a novel microstructure and ion-releasing profile, the cytotoxicity of 3DOM-BG particles was tested. The cytotoxicity tests were performed using MC3T3-E1 osteoblast-like cells: (1) Wst-1 assay for cell viability after culture in extracts from 3DOM-BG particles; (2) phase contrast microscopy for cell morphology after culture with 3DOM-BG particles; and (3) fluorescence microscopy for imaging cells cultured directly on 3DOM-BG particles. The results showed that 3DOM-BG particles were not cytotoxic, and that cells attached, spread and proliferated on and around 3DOM-BG particles.
|
| Volume |
26(22)
|
| Pages |
4532-9
|
| Published |
2005-8-1
|
| DOI |
10.1016/j.biomaterials.2004.11.030
|
| PII |
S0142-9612(04)01012-9
|
| PMID |
15722122
|
| MeSH |
3T3 Cells
Animals
Biocompatible Materials*
Cell Survival*
Glass*
Mice
Microscopy, Fluorescence
|
| IF |
10.317
|
| Times Cited |
28
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
