RRC ID 42338
Author Ku Y, Chung CP, Jang JH.
Title The effect of the surface modification of titanium using a recombinant fragment of fibronectin and vitronectin on cell behavior.
Journal Biomaterials
Abstract The surface of titanium implants is in direct contact with host tissue and plays a critical role in determining biocompatibility. Fibronectin (FN) and vitronectin (VN) are major cell adhesive proteins found in the extracellular matrix (ECM) of various tissues, and in circulating blood. The aim of this study was to evaluate the engineered biomimetic surface of titanium by using recombinant fragment of FN(8-10) and VN(NTD) that contains the binding site for integrins. MC3T3-E1 cells seeded upon the FN(8-10)-coated titanium showed a marked increase in cell adhesion, proliferation, and differentiation over VN(NTD)-coated titanium. In addition, we confirmed that the surface properties of titanium prefer for FN(8-10) over VN(NTD) (p<0.05) in protein adhesion. These results suggest that the FN(8-10)-modified titanium surface can be used to improve the osseointegration of titanium implants by enhancing bone formation.
Volume 26(25)
Pages 5153-7
Published 2005-9-1
DOI 10.1016/j.biomaterials.2005.01.060
PII S0142-9612(05)00110-9
PMID 15792542
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Biomimetic Materials / chemistry Biomimetic Materials / pharmacology Cell Adhesion / drug effects Cell Differentiation / drug effects Cell Proliferation / drug effects Coated Materials, Biocompatible / pharmacology* DNA-Binding Proteins / genetics Fibronectins / genetics* Gene Expression / genetics Humans Mice Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism Peptide Fragments / chemistry Peptide Fragments / metabolism Peptide Fragments / pharmacology* Protein Binding Recombinant Proteins / chemistry Recombinant Proteins / isolation & purification Recombinant Proteins / pharmacology Surface Properties Titanium / chemistry* Titanium / metabolism Transcription Factor AP-2 Transcription Factors / genetics Vitronectin / genetics*
IF 10.317
Times Cited 63
Human and Animal Cells MC3T3-E1(RCB1126)