RRC ID 42347
Author El Bohi KM, Sabik L, Muzandu K, Shaban Z, Soliman M, Ishizuka M, Kazusaka A, Fujita S.
Title Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of Salmonella typhimurium TA98 elicited by benzo[a]pyrene and oxidative DNA lesions in V79 hamster lung cells.
Journal Jpn J Vet Res
Abstract Pleurotus cornucopiae (PC) mushroom with a brilliant yellow pileus is found in the field and known in Japan as Tamogi dake mushroom. The purpose of this paper is to investigate the mechanism of the antimutagenic effect of PC mushroom using both the Ames test and Comet assay. We have found a strong inhibitory effect of both aqueous and organic PC extracts on the mutagenicity elicited by benzo[a]pyrene (B[a]P). This inhibition was dose-dependent in reaction mixtures containing cytosolic and microsomal fractions (S-9) from untreated rat liver as well as in those containing S-9 from aryl hydrocarbon receptor (Ah) ligand of Sudan III-treated rats. Sudan III was a potent inducer of cytochrome P450 1A (CYP1A) activity. We treated rats with Sudan III to enhance the metabolic activation of B[a]P by the S-9 fraction. To explain whether this antimutagenicity was due to the inhibition of CYP1A activity that metabolically activates B[a]P, we tested the effects of the extracts on activities of CYP1A1 and CYP1A2, represented by ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD), respectively. Both aqueous and organic extracts inhibited EROD activity at all dose levels, while the inhibitory effect was only observed at high doses with regard to MROD activity. Furthermore, pre-treatment of Chinese hamster V79cells with PC extracts significantly reduced H2O2-induced-DNA damage, indicating that PC extracts provide a protective effect against oxidative DNA damage. These results indicate that whole-mushroom extracts contain compounds that may inhibit the metabolic activation of B[a]P by CYP1A1 as well as prevent oxidative DNA damage.
Volume 52(4)
Pages 163-72
Published 2005-2-1
PMID 15822858
MeSH Animals Antimutagenic Agents* Benzo(a)pyrene / antagonists & inhibitors* Benzo(a)pyrene / toxicity Biological Products / pharmacology Cell Line Cricetinae Cricetulus DNA Damage* / drug effects Hydrogen Peroxide / antagonists & inhibitors Mutagenicity Tests Pleurotus* Salmonella typhimurium / drug effects Salmonella typhimurium / genetics*
IF 0.323
Times Cited 11
Human and Animal Cells V79(RCB0008)