| RRC ID |
42365
|
| Author |
Yu J, Leung WK, Chen J, Ebert MP, Malfertheiner P, Sung JJ.
|
| Title |
Expression of peroxisome proliferator-activated receptor delta in human gastric cancer and its response to specific COX-2 inhibitor.
|
| Journal |
Cancer Lett
|
| Abstract |
Peroxisome proliferator-activated receptor delta (PPARdelta) is ligand-activated transcription factor of the nuclear receptor superfamily which is recently implicated in carcinogenesis. We examined the expression profiles of PPARdelta in human gastric cancer, normal gastric mucosa and gastric cancer cell lines by RT-PCR, Western blot and immunohistochemistry. PPARdelta mRNA and protein was found to be ubiquitously expressed in all 5 gastric cancer cell lines, 40 gastric cancer samples and 10 normal gastric mucosa from non-cancer patients. Positive immunoreactivity was detected in the nuclei of normal and malignant gastric epithelium. Treatment of gastric cell line MKN45 that overexpressed cyclooxygenase-2 (COX-2) with specific COX-2 inhibitor NS398 resulted in a time- and dose-dependent suppression of PPARdelta expression. In contrast, there was no suppression of PPARdelta in MKN28 gastric cell line with low COX-2 expression. Our results demonstrated the ubiquitous expression of PPARdelta in normal and cancer gastric epithelium. Suppression of PPARdelta may be one of the mechanisms underlying the chemopreventive effects of COX-2 inhibitor.
|
| Volume |
223(1)
|
| Pages |
11-7
|
| Published |
2005-6-1
|
| DOI |
10.1016/j.canlet.2004.09.052
|
| PII |
S0304-3835(04)00771-2
|
| PMID |
15890232
|
| MeSH |
Adult
Aged
Cell Line, Tumor
Cyclooxygenase Inhibitors / pharmacology*
Female
Humans
Male
Middle Aged
PPAR gamma / analysis
PPAR gamma / antagonists & inhibitors*
PPAR gamma / genetics
Stomach Neoplasms / chemistry*
Stomach Neoplasms / metabolism
|
| IF |
7.36
|
| Times Cited |
9
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
|
