Reference - Detail
RRC ID | 42367 |
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Author | Hattori Y, Maitani Y. |
Title | Folate-linked nanoparticle-mediated suicide gene therapy in human prostate cancer and nasopharyngeal cancer with herpes simplex virus thymidine kinase. |
Journal | Cancer Gene Ther |
Abstract |
For targeted gene delivery to human prostate cancer LNCaP and PC-3 cells and nasopharyngeal cancer KB cells, we developed a folate-linked nanoparticle (NP-F), and evaluated the potential of NP-F-mediated suicide gene therapy in the cells and xenografts with herpes simplex virus thymidine kinase (HSV-tk) and connexin 43 (Cx43). An NP-F-plasmid DNA complex (NP-F nanoplex) showed high DNA transfection efficiency in KB, LNCaP and PC-3 cells. Cell growth inhibition in the presence of ganciclovir (GCV) was enhanced with HSV-tk and Cx43 genes in LNCaP cells. In suicide gene therapy, the tumor growths of KB and LNCaP xenografts were significantly inhibited when an NP-F nanoplex of the HSV-tk gene, and HSV-tk and Cx43 genes, respectively, was injected intratumorally and GCV was administered intraperitoneally. These findings suggested that the NP-F is a potential target vector in prostate and nasopharyngeal cancer for suicide gene therapy. |
Volume | 12(10) |
Pages | 796-809 |
Published | 2005-10-1 |
DOI | 10.1038/sj.cgt.7700844 |
PII | 7700844 |
PMID | 15891776 |
MeSH | Chromatography, High Pressure Liquid Connexin 43 / metabolism DNA Primers Flow Cytometry Fluorescein-5-isothiocyanate Folic Acid / metabolism* Gene Transfer Techniques Genes, Transgenic, Suicide / genetics* Genetic Therapy / methods* Genetic Vectors / genetics Humans Immunohistochemistry Luciferases Male Nanostructures* Nasopharyngeal Neoplasms / therapy* Plasmids / genetics Prostatic Neoplasms / therapy* Simplexvirus / genetics Simplexvirus / metabolism* Thymidine Kinase / genetics Thymidine Kinase / metabolism* |
IF | 4.534 |
Times Cited | 77 |
WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY ONCOLOGY |
Resource | |
Human and Animal Cells | Hep G2 |