論文 - 詳細
| RRC ID | 42367 |
|---|---|
| 著者 | Hattori Y, Maitani Y. |
| タイトル | Folate-linked nanoparticle-mediated suicide gene therapy in human prostate cancer and nasopharyngeal cancer with herpes simplex virus thymidine kinase. |
| ジャーナル | Cancer Gene Ther |
| Abstract |
For targeted gene delivery to human prostate cancer LNCaP and PC-3 cells and nasopharyngeal cancer KB cells, we developed a folate-linked nanoparticle (NP-F), and evaluated the potential of NP-F-mediated suicide gene therapy in the cells and xenografts with herpes simplex virus thymidine kinase (HSV-tk) and connexin 43 (Cx43). An NP-F-plasmid DNA complex (NP-F nanoplex) showed high DNA transfection efficiency in KB, LNCaP and PC-3 cells. Cell growth inhibition in the presence of ganciclovir (GCV) was enhanced with HSV-tk and Cx43 genes in LNCaP cells. In suicide gene therapy, the tumor growths of KB and LNCaP xenografts were significantly inhibited when an NP-F nanoplex of the HSV-tk gene, and HSV-tk and Cx43 genes, respectively, was injected intratumorally and GCV was administered intraperitoneally. These findings suggested that the NP-F is a potential target vector in prostate and nasopharyngeal cancer for suicide gene therapy. |
| 巻・号 | 12(10) |
| ページ | 796-809 |
| 公開日 | 2005-10-1 |
| DOI | 10.1038/sj.cgt.7700844 |
| PII | 7700844 |
| PMID | 15891776 |
| MeSH | Chromatography, High Pressure Liquid Connexin 43 / metabolism DNA Primers Flow Cytometry Fluorescein-5-isothiocyanate Folic Acid / metabolism* Gene Transfer Techniques Genes, Transgenic, Suicide / genetics* Genetic Therapy / methods* Genetic Vectors / genetics Humans Immunohistochemistry Luciferases Male Nanostructures* Nasopharyngeal Neoplasms / therapy* Plasmids / genetics Prostatic Neoplasms / therapy* Simplexvirus / genetics Simplexvirus / metabolism* Thymidine Kinase / genetics Thymidine Kinase / metabolism* |
| IF | 4.534 |
| 引用数 | 77 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |