Reference - Detail
| RRC ID | 42368 |
|---|---|
| Author | Matsumoto S, Saito H, Tsujitani S, Ikeguchi M. |
| Title | Allogeneic gastric cancer cell-dendritic cell hybrids induce tumor antigen (carcinoembryonic antigen) specific CD8(+) T cells. |
| Journal | Cancer Immunol Immunother |
| Abstract |
The development of protocols for the ex vivo generation of dendritic cells (DCs) has led to intensive research of their potential use in immunotherapy. Accumulating results show the efficacy of this treatment on melanomas which are highly immunogenic. However, its efficacy remains unclear in other tumors. In this study, allogeneic gastric cancer cell-DC hybrids were used to determine the efficacy of this type of immunotherapy in gastric cancer. Fusion cells of DC and allogeneic gastric cancer cells were generated by polyethylene glycol (PEG) and electrofusion. These hybrids were used to induce tumor associated antigen (TAA) specific cytotoxic T lymphocytes (CTLs). The DCs were successfully fused with the allogeneic gastric cancer cells resulting in hybrid cells. These hybrid cells were functional as antigen-presenting cell because they induced allogeneic CD4(+) T cells proliferation. CD8(+) T cells stimulated by the MKN-45-DC hybrid cells were able to kill MKN-45 when used for immunization. The CTLs killed another gastric cancer cell line, MKN-1, as well as a melanoma cell line, 888mel, suggesting the recognition of a shared tumor antigen. MKN-45 specific CTLs can recognize carcinoembryonic antigen (CEA), indicating that the killing is due to tumor antigens as well as alloantigens. This approach suggests the possible use of allogeneic gastric cancer cell-DC hybrids in DC based immunotherapy for gastric cancer treatment. |
| Volume | 55(2) |
| Pages | 131-9 |
| Published | 2006-2-1 |
| DOI | 10.1007/s00262-005-0684-3 |
| PMID | 15891883 |
| MeSH | CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / cytology* CD8-Positive T-Lymphocytes / immunology* Carcinoembryonic Antigen / immunology* Cell Fusion Cell Line, Tumor Cell Proliferation Dendritic Cells / immunology* Electric Stimulation Humans Hybrid Cells / cytology Hybrid Cells / immunology* Isoantigens / immunology Stomach Neoplasms / immunology Stomach Neoplasms / therapy* T-Lymphocytes, Cytotoxic / immunology |
| IF | 5.442 |
| Times Cited | 9 |
| WOS Category | IMMUNOLOGY ONCOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MKN1(RCB1003) MKN45(RCB1001) K562 |