| RRC ID |
42371
|
| 著者 |
Iwasaki Y, Tabata E, Kurita K, Akiyoshi K.
|
| タイトル |
Selective cell attachment to a biomimetic polymer surface through the recognition of cell-surface tags.
|
| ジャーナル |
Bioconjug Chem
|
| Abstract |
Reactive phosphorylcholine polymers, which can recognize biosynthetic cell-surface tags, were synthesized to control cell attachment. Human promyelocytic leukemia cells (HL-60) with unnatural carbohydrates as cell-surface tags were harvested by treatment with N-levulinoylmannosamine (ManLev). The attachment of ManLev-treated HL-60 cells to 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers with hydrazide groups was studied. HL-60 cells, which are nonadhesive, did not attach to any polymer surface without ManLev treatment. In contrast, ManLev-treated HL-60 cells attached to a poly[MPC-co-n-butyl methacrylate (BMA)-co-methacryloyl hydrazide (MH)] (PMBH) surface following 15 min of incubation. The cells that attached to the PMBH surface retained their native morphology and viability for 24 h of incubation. On the other hand, approximately half of the HL-60 cells that attached to the poly(BMA-co-MH) (PBH) surface died. These results suggest that MH units in the polymer act as anchors for cell attachment and MPC units help to preserve cell viability on a polymer surface. The coculture of ManLev-treated HL-60 and fluorescence-stained human uterine cervical cancer cells (HeLa) was carried out on polymer surfaces. ManLev-treated HL-60 cells specifically attached to the PMBH surface. In contrast, both HL-60 and HeLa cells were observed on the PBH surface. The control of cellular interactions with synthetic polymers may be useful for the future development of cell-integrated biosensors and biomedical devices.
|
| 巻・号 |
16(3)
|
| ページ |
567-75
|
| 公開日 |
2005-1-1
|
| DOI |
10.1021/bc049707r
|
| PMID |
15898723
|
| MeSH |
Adsorption
Biomimetic Materials / chemical synthesis
Biomimetic Materials / chemistry*
Biomimetic Materials / toxicity
Cell Adhesion
Cell Survival
Coculture Techniques
Fibronectins / metabolism
HL-60 Cells
HeLa Cells
Humans
Microscopy, Fluorescence
Molecular Structure
Neuraminidase / metabolism
Polymers / chemical synthesis
Polymers / chemistry*
Polymers / toxicity
Sensitivity and Specificity
Spectrum Analysis
|
| IF |
4.031
|
| 引用数 |
25
|
|
WOS 分野
|
CHEMISTRY, MULTIDISCIPLINARY
CHEMISTRY, ORGANIC
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
