| RRC ID |
42373
|
| Author |
Cheng CM, Tsuneyama K, Matsui K, Takahashi H, Ishizawa S, Takano Y.
|
| Title |
Cytoplasmic expression of c-erbB2 in non-small cell lung cancers.
|
| Journal |
Virchows Arch
|
| Abstract |
The aim of this study was to investigate the expression of c-erbB2 in non-small cell lung cancers (NSCLCs) with attention both to membranous and cytoplasmic reaction, and to try to elucidate the meaning of cytoplasmic expression of c-erbB2 in NSCLCs. Immunohistochemical c-erbB2 expression and related clinico-pathological features were examined in 312 surgically resected patient tissues of NSCLCs, including 175 cases of adenocarcinoma and 137 cases of squamous cell carcinoma. Immunostaining of inner- and ecto-domain of c-erbB2, mRNA expression and the quantitation of soluble c-erbB2 in cultured media were performed in five NSCLC cell lines. Cytoplasmic expression of c-erbB2 was observed more frequently than membranous, both in patient tissues and cell lines. Neither membranous nor cytoplasmic expression of c-erbB2 was significantly correlated with short outcome in NSCLCs. Membranous c-erbB2 was expressed by both inner and ecto-domain, while cytoplasmic c-erbB2 was expressed by either or both inner and ecto-domain. c-erbB2 mRNA was produced in most cell lines; however, the soluble form was only detectable in a cell line that only presented a membranous c-erbB2. In conclusion, cytoplasmic c-erbB2 of NSCLCs was not a full-length protein only expressed in cellular membrane, but reflected degenerated c-erbB2 fragments with less functional ability.
|
| Volume |
446(6)
|
| Pages |
596-603
|
| Published |
2005-6-1
|
| DOI |
10.1007/s00428-005-1258-7
|
| PMID |
15902485
|
| MeSH |
Adult
Aged
Aged, 80 and over
Carcinoma, Non-Small-Cell Lung / metabolism*
Carcinoma, Non-Small-Cell Lung / mortality
Cell Line, Tumor
Cell Membrane / metabolism*
Cytoplasm / metabolism*
Female
Humans
Immunoenzyme Techniques
Immunohistochemistry
Lung Neoplasms / metabolism*
Lung Neoplasms / mortality
Male
Middle Aged
Receptor, ErbB-2 / biosynthesis*
Survival Analysis
|
| IF |
2.906
|
| Times Cited |
10
|
|
WOS Category
|
PATHOLOGY
|
| Resource |
| Human and Animal Cells |
LC-1/sq(RCB0455)
LC-1F(RCB0439) |
